Effluents from wastewater treatment works (WwTW) exhibit both temporal and spatial variation in oestrogenicity, however few studies have attempted to quantify how this affects biological responses fish. Here we used an oestrogen-responsive green fluorescent protein (ERE-GFP) transgenic zebrafish (Danio rerio) oestrogenic activity health effects for exposure three different WwTW effluents. Endpo...

Populations of killifish (Fundulus heteroclitus) persist in many different highly polluted environment indicative of adaptation or tolerance. In this study, we sought to determine whether long term, multigenerational exposures to environmental contaminants has affected reproductively relevant genes and biological processes. A homology cloning strategy was used to isolate the killifish cytochrom...

The EU-funded project IDEA aimed to evaluate (a) what parameters and endpoints allow the detection of endocrine-mediated developmental and reproductive effects of (xeno)estrogens in life cycle- and life stage-specific toxicity tests with the zebrafish Danio rerio, a small laboratory fish used in many ecotoxicity test guidelines, and (b) whether substances that act as estrogens in vertebrates ma...

Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) and triphenyl phosphate (TPP) belong to the group of triester organophosphate flame retardants (OPFRs), which have been used in a wide range of consumer products. These chemicals have been frequently detected in effluents, surface water, and fish, and hence their potential adverse effects on aquatic ecosystem are of concern. The present study was co...

To accommodate the next generation of adaptive/variable cycle engines and gas turbine power, variable geometry (VTG) is widely acknowledged as a most essential component. VGT consists an adjustable vane to address combined goals high dry thrust low specific fuel consumption (SFC) at subsonic cruises for aero-engines. This concept allows engine operate constant bypass ratio over wide range press...

Bisphenol A (BPA) is a synthetic compound widely used in types of consumer goods and medical tools. It has been shown that BPA acts as an endocrine disruptor chemical casuing negative impact on reproductive functions. The present study used an in vitro approach to assess estrogenic transcriptional activity of BPA as a rapid and sensitive method. The cultured pieces of hepatic tissue of goldfish...

Transgenic zebrafish models are efficiently used to study the effects of endocrine disrupting chemicals (EDC); thereby informing on their mechanisms action. However, given reported differences between strains at genetical, physiological and behavioral levels; care should be taken before using these transgenic for EDC testing. In present study, we undertook a set experiments in different and/or ...

Case presentation: VTG, 2 years old, daughter of non-consanguineous parents, born by cesarean section at 35 weeks. At birth, he had neonatal sepsis and hypoglycemia. 3 months, started generalized tonic-clonic seizures associated with behavioral arrest ocular version, refractory to treatment optimization antiepileptic drugs. In addition neuropsychomotor developmental regression, sleep disturbanc...