in this study, srap molecular maker approach was performed to investigate genetic diversity in the salvia genus. a total of 205 dna bands were produced from pcr amplification of 11 salvia species and populations using 25 selective primer combinations, of which 204 polymorphic genetic loci accounted. the total number of amplified fragments ranged from 3 to 15. the genetic similarities of 11 coll...

chitosan/polyethersulfone (cs/pes) composite membrane was prepared from casting chitosan solution on polyethersulfone substrate membrane. the substrate membrane was prepared by phase inversion technique using polyethersulfone (pes) and dimethylacetamide (dmac) as solvent with and without the addition of polyvinylpyrrolidone (pvp) as pore-forming agent. the effects of the composition of the c...

spinal muscular atrophy (sma) is a genetic disorder which affect nervous system and is characterized with progressive distal motor neuron weakness. the survival motor neuron (smn) protein level reduces in patients with sma. two different genes code survival motor neuron protein in human genome. skeletal and intercostal muscles denervation lead to weakness, hypotony, hyporeflexia, respiratory fa...

G3 rotaviruses have been reported rarely in cattle, and none have been characterized. We report the first genomic characterization of a bovine G3 rotavirus, CP-1, which had been biologically characterized in vivo and shown to cause age-independent diarrhea. CP-1 was a G3 rotavirus as its VP7 had 92 to 96% deduced amino acid identity to those of G3 rotaviruses. However, initially, CP-1 was ident...

Between 1998 and 2000, a total of 266 samples were found positive for group A rotaviruses by RNA electrophoresis. Samples were collected from patients admitted to two leading hospitals in Calcutta. Serotyping could be done only with 22% of the positive samples, leaving 78% untypeable. The G (VP7 genotypes) and P (VP4 genotypes) types were determined for 159 samples by reverse transcription and ...

The complete sequence of the S7 RNA that codes for the major group-specific coat protein. VP7, of African horsesickness virus serotype 4 (AHSV-4) was determined from cDNA analyses and found to be 1179 nucleotides in length. One single open reading frame of 353 codons was observed defining a protein of Mr 38,107 with a net charge of -1.5 at neutral pH. Comparison of the AHSV-4 VP7 sequence with ...

During 2001, an outbreak of severe acute gastroenteritis swept through Central and northern Australia and caused serious disruption to health services. We tracked and characterized the rotavirus strain implicated in the outbreak. Comparison of the electropherotypes of outbreak samples suggested that one G9P[8] strain was likely responsible for the outbreak. Samples were obtained from geographic...

Increasing numbers of studies have documented the widespread distribution of human G9 rotaviruses and demonstrated that these strains may represent a fifth epidemiologically important G serotype. Serotype G9 strains were identified in Hungary for the first time in the 1997-1998 rotavirus season. Contrary to numerous surveys that reported several unexpected P-G combinations among recent G9 isola...

Rotavirus remains a major cause of diarrhea in infants and young children especially in developing countries. Monitoring changes in rotavirus strains is necessary to assess the potential effectiveness of vaccines in specific geographic locations. This project aimed at monitoring the epidemiological status of rotavirus among Thai children. A total of 73 positive fecal samples for rotavirus were ...

Precise investigation of genetic diversity by means of novel molecular tools has made it possible to identify the superior genotypes among various male and female pistachio populations. Cytogenetic studies have shed light on the possible presence of distinct sex chromosomes in male and female genotypes. In this study, 22 start codon targeted (SCoT) primers were used to investigate the genetic d...