AIMS To compare prospectively the efficacy of the Vero, LLC-MK2, MDCK, Hep-2, and MRC-5 cell lines in the isolation of the mumps virus from clinical samples by means of the shell vial method. METHODS During an epidemic outbreak of parotiditis 48 clinical samples (saliva swabs and CSF) were studied. Two vials of the Vero, LLC-MK2, MDCK, MRC-5, and Hep-2 cell lines were inoculated with 0.2 ml o...

foot and mouth disease (fmd) is highly contagious disease among cloven-hoofed animals. fmd virus has structural and non-structural proteins. vp1 is the most immunogenic structural peptides of fmd virus, applied for major vaccine studies. objective: construction of pet28-vp1 cassette for fmd virus type o/irn/2010 and expression vp1 peptide as the most immunogenic antigen was the aim of this stud...

food borne viral infections are recognized increasingly as human illneses. hepatitis a virus (hav) is an important pathogen which has been responsible for water and many food – borne outbreeks especially milk . in this study for approaching to best diagnosis method of hav in milk by elisa technique, various dilutions of hav antigen (1 ml/l, 10-3 ml/l, 10-6 ml/l, 10-9 ml/l) were added to uht mil...

• Additional tests are available to actually determine the presence of the virus. These tests include immunohistochemistry staining (IHC), fluorescent antibody staining (FA), polymerase chain reaction (PCR), and virus isolation (VI). While a positive result on these tests indicates the virus is present in the sample, a negative test does not necessarily indicate that the pig is free of the viru...

Results: Larger herd size was associated with an increased risk, and isolation of purchased gilts with a decreased risk, of both PRRS virus infection and clinical PRRS. Purchase of semen for AI was associated with an increased risk of PRRS virus infection. Implications: Swine producers should certify that purchased semen is free of PRRS virus, gilts should be isolated prior to introduction to t...

CONTEXT Preventing nosocomial transmission of influenza is essential to reduce the morbidity and mortality associated with this infection. In October 2009, an outbreak of the 2009 influenza A (H1N1) virus occurred in a hematology ward of a children's hospital over a 21-day period and involved two patients and four healthcare workers. OBJECTIVE To investigate nosocomial transmission of the 200...

Pork products are a possible source of introduction of PCV2 isolates into a pig population. However, limited work has been done on the transmission through meat of porcine circovirus type 2 (PCV2), a virus associated with several disease syndromes in pigs. The objectives of this study were to determine if pork products from PCV2-infected pigs contain PCV2 DNA/antigen and to determine if the PCV...

We report a comparative study of the MDCK, Vero, and MRC-5 cell lines in the isolation of the influenza A (IA) virus. We studied 746 samples in which 63 IA viruses were isolated. The MDCK line displayed 100% sensitivity, the Vero line displayed 71.4%, and the MRC-5 displayed 57.1%. The MDCK line showed a statistically significant difference with respect to the Vero line (P = 0.001) and the MRC-...

Parasites and pathogens are apparent key factors for the detrimental health of managed European honey bee subspecies, Apis mellifera. Apicultural trade is arguably the main factor for the almost global distribution of most honey bee diseases, thereby increasing chances for multiple infestations/infections of regions, apiaries, colonies and even individual bees. This imposes difficulties to eval...

Lumpy skin disease is characterized by its signs including eruptive, infectious and occasionally causing death to affected animals. It caused the family Poxviridea genus Capripox with a strain of Neethling virus, double stranded DNA virus. LSD clinical are mainly observed on as nodules (0.5 5 cm in diameter) throughout surface or subcutaneous tissue involving superficial lymph nodes. Diagnosis ...