Potato spindle tuber viroid (PSTVd) is a harmful quarantine disease with wide geographic distribution. To date, experimentally proved resistance or tolerance of potato cultivars to PSTVd has not been reported. The aim this study was evaluate responses four strains 39 modern different origin. Four origin, the intermediate VP35, VP87, and two sever FP10-13 NicTr-3, deposited in GenBank, were used...

Abstract Citrus exocortis is a grafting disease caused by Exocortis Viroid (CEVd). The knowledge of the viroid’s incidence and distribution are necessary to further apply control measures. objective this work was real time PCR assay for detection CEVd in samples collected from symptomatic CEVd-infected plants Mitidja (North Algeria). showed an excellent diagnostic specificity where 38 out 50 po...

background and aims: plant certification programs need reliable, fast, cheap and sensitive methods for detection of systemic pathogens with special interest in virus and viroid detection. reverse transcriptase-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certification of plant propagating materials. the main object of the present study was the optimization ...

Background and Aims: Viroids are smallest known plant pathogens and cause several economically significant diseases. Until recently, viroid detection relied mainly on biological tests and indexing. Today various diagnostic techniques such as nucleic acid hybridization, southern blot and reverse transcription coupled with polymerase chain reaction (RT-PCR) are being used for detection and diag...