We compared a fluorescein-labeled monoclonal antibody directed against an outer membrane protein of Legionella pneumophila (Genetic Systems Corp. [GSC], Seattle, Wash.) with a similarly labeled polyclonal reagent (L. pneumophila serogroups 1 to 6, poly; BioDx, Inc., Denville, N.J.) for the confirmation of L. pneumophila isolates grown in culture. Duplicate suspensions of 52 organisms, including...

I examined a cholesterol-assay kit ("Autoflo", Biodynamics/bmc) designed for use with continuous-flow instrumentation for its use with some discrete analyzers. At pH 7.2, color develops within 3 min upon exposure to cholesterol, then begins to fade. At pH 8, the color develops more slowly but remains stable for at least 15 min after reaching a maximum. A pH 8.8, the color develops still more sl...

This study was aimed to evaluate the efficacy of dipstick tests (leukocyte esterase and nitrite) in diagnosis of spontaneous bacterial peritonitis (SBP) in cirrhotic patients. Forty six children with ascites hospitalized between 2009 and 2010 in Children Medical Center were enrolled in this study. Reagent strip assays for leukocyte esterase and nitrite were performed on ascetic fluid and the re...

Hitachi704 Unmodified No 155 y 1.OOlx + 0.23 0.988 0.0118 0.225 0.29 Unmodified Yes 52 y = 1.059x + 9.71 0.987 0.0234 0.453 10.71 b Modified Yes 155 y = 1.047x 0.50 0.996 0.0074 0.142 0.21 Hitachi 717 Unmodified No 155 y = 1.025x + 0.14 0.977 0.0213 0.425 0.57 Unmodified Yes 31 y = 1.040x + 3.20 0.960 0.0522 1.283 406b Modified Yes 155 y = 1.065x 0.19 0.983 0.01 89 0.380 Three sets of samples w...

Acetophenone 2’, 4’dihydroxy semicarbazone [A24DHS], a new analytical reagent is proposed as a sensitive and selective spectrophotometric reagent for Vanadium (V). The reagent A24DHS is synthesized in the laboratory and characterized by NMR, IR and elemental analysis. A selective spectrophotometric method is presented for the trace determination of Vanadium using A24DHS as spectrophotometric re...

Preparation of oligoribonucleotides with a phosphorothioate backbone requires a sulfurizing reagent that is efficient and stable in solution. One of the most efficient sulfurizing reagents, 3H1,2-benzodithiol-3-one-1,1-dioxide (Beaucage Reagent), is stable in solution but lacks longterm stability on the DNA synthesizer. This report evaluates Beaucage Reagent and 3((N,N-dimethyl-aminomethylidene...

A new microcrystal test for the detection of gamma-hydroxybutyrate (GHB) is described. The silver/copper reagent consists of an aqueous solution of 0.1 g of cupric nitrate and 0.1 g of silver nitrate in 10.0 mL water. While some crystals form upon evaporation of the reagent, the test forms distinctive crystals for GHB and does not form crystals with some commonly encountered controlled substanc...