A real-time PCR assay previously developed for use on the Roche LightCycler platform was investigated as an alternative to culture for the direct detection of vancomycin-resistant enterococci (VRE) in clinical specimens. PCR primers and fluorescence resonance energy transfer hybridization probes specific for the vanA and vanB genes were combined in a multiplex real-time PCR assay performed dire...

BACKGROUND Vancomycin-resistant enterococci (VRE) are important nosocomial pathogens and food chain has been considered as an assumed source for dissemination of VRE to human. OBJECTIVES The presence of VRE isolates from food samples and typing of these isolates with Phene plate, a biochemical fingerprinting method, were investigated. MATERIALS AND METHODS Thirty samples of meat, chicken an...

The goal of this study was to assess the presence of enterococci species presenting van-mediated glycopeptide resistance in French cattle. Fecal samples were collected from healthy and sick animals, and enterococci were screened for vancomycin resistance. Vancomycin resistance was principally encountered in Enterococcus gallinarum and Enterococcus casseliflavus strains. However, glycopeptide re...

A set of well characterized strains, collected in Polish hospitals, including Gram-negative (n = 93) and Gram-positive (n = 90) isolates was used in the study. The VITEK 2 AST-cards were used in the analysis according to the manufacturer's recommendations. Comparison of the susceptibility data obtained by the standard method and by VITEK 2 cards proved concordant in 99% of cases. Clinically imp...

The World Health Organization (WHO) has defined Vancomycin intermediate Staphylococcus aureus (VISA) and resistant (VRSA) as high priority pathogens. VISA VRSA are produced by different mechanisms, hence cannot convert to VRSA. Consequently, upon vancomycin treatment of VSSA isolates, there can be emergence but not its conversion But we observed that when (MIC ≤2 µg/mL) lacking vanA or vanB gen...

conclusions: the combination of plasmid profiling and php techniques revealed the presence of diverse population of vre in sewage treatment plant in tehran. furthermore, the results showed that the php technique is a reliable method in determining the vre clonal diversity. results: all isolates were found to be resistant to erythromycin, ampicillin and ciprofloxacin. the pcr analyses showed tha...

background and objectives: infection with vancomycin-resistant enterococci (vre) has caused a therapeutic problem. vana and vanb resistant types are the predominant phenotypes among vancomycin resistant enetrococci. transposon 1546 (tn1546) harboring the vana gene cluster, plays an important role in the horizontal transfer of vana gene. in this study, we examined the phenotypic and genotypic di...