Mutagenesis is an important tool for breeding and genomic research. In this study, the germinated seeds isolated microspores of a double haploid line ‘FT’ were treated with EMS, respectively, aim comparing effects two approaches on generating mutants in Chinese cabbage. For microspore EMS mutagenesis, 0.12% 20 min, total 1268 plantlets obtained, 15 M1 screened mutation frequency 1.2%. seed 7800...

Mutagenesis in Escherichia coli, a subject of many years of study is considered to be related to DNA replication. DNA lesions nonrepaired by the error-free nucleotide excision repair (NER), base excision repair (BER) and recombination repair (RR), stop replication at the fork. Reinitiation needs translesion synthesis (TLS) by DNA polymerase V (UmuC), which in the presence of accessory proteins,...

Random mutagenesis is a useful tool to genetically modify organisms for various purposes, such as adaptation to cultivation conditions, the induction of tolerances, or increased yield of valuable substances. This is especially attractive for systems where it is not obvious which genes require modifications. Random mutagenesis has been extensively used to modify crop plants, but even with the re...

Objective(s):[p1]  This study highlights xylanase overproduction from Bacillus mojavensis via UV mutagenesis and optimization of the production process. Materials and Methods:Bacillus mojavenis PTCC 1723 underwent UV radiation. Mutants’ primary screening was based on the enhanced Hollow Zone Diameter/ Colony Diameter Ration (H/C ratios) of the colonies in comparison with the wild strain on Xyla...

Abstract We present TensorSignatures, an algorithm to learn mutational signatures jointly across different variant categories and their genomic localisation properties. The analysis of 2778 primary 3824 metastatic cancer genomes the PCAWG consortium HMF cohort shows that all operate dynamically in response states. pins differential spectra UV mutagenesis found active inactive chromatin global g...

We have previously shown that CK2 associates with the human high-mobility group protein SSRP1 and that this association increases in response to UV irradiation. CK2 also phosphorylates SSRP1 in vitro. Here we extend this work by investigating CK2 regulation of SSRP1 function through phosphorylation. Phosphorylation of SSRP1 by CK2 inhibited the nonspecific DNA-binding activity of SSRP1 and FACT...

During the end-Permian ecological crisis, terrestrial ecosystems experienced preferential dieback of woody vegetation. Across the world, surviving herbaceous lycopsids played a pioneering role in repopulating deforested terrain. We document that the microspores of these lycopsids were regularly released in unseparated tetrads indicative of failure to complete the normal process of spore develop...