نتایج جستجو برای: ura
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Candida albicans ERG3 encodes a sterol C5,6-desaturase which is essential for synthesis of ergosterol. Defective sterol C5,6 desaturation has been considered to be one of the azole resistance mechanisms in this species. However, the clinical relevance of this resistance mechanism is still unclear. In this study, we created a C. albicans erg3/erg3 mutant by the "Ura-blaster" method and confirmed...
A three-plasmid yeast expression system utilizing the portable small ubiquitin-like modifier (SUMO) vector set combined with the efficient endogenous yeast protease Ulp1 was developed for production of large amounts of soluble functional protein in Saccharomyces cerevisiae. Each vector has a different selectable marker (URA, TRP, or LEU), and the system provides high expression levels of three ...
The advanced cloning-free PCR-based allele replacement strategy (Erdeniz et al., 1997) has been followed in the present study. The principle of this strategy is graphically depicted in Figure 1a. In the gene targeting experiment performed in the present study, the uracil auxotrophy (Ura-) of CEN.PK 113-5D strain has been exploited. Thus, cells where the gene targeting substrate integrated corre...
SPECTRAL ELEMENT METHOD PART 1: NUMERICAL ALGORITHMS Aimé Fournier, Joseph Tribbia National Center for Atmospheri Resear h Boulder CO 80307-3000 USA fournier u ar.edu Mark A. Taylor Albuquerque NM 87108 USA www.s d.u ar.edu/ ss/staff/taylor ABSTRACT It is well known that the fast and a urate solution of the partial di erential equations (PDEs) governing geophysi al uid dynami s presents a great...
0 Introduction Backward Stochastic Diierential Equations (in short BSDE's) are new type of stochastic diierential equations, whose terminal value is a given random variable, which have been introduced in particular by Pardoux, Peng in 5]. They have proved to be useful models in Mathematical Finance, see Bar-les, Buckdahn, Pardoux 2] and the references therein. In 6], it has been show that BSDE'...
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