Large-scale deletions of mitochondrial DNA (mtDNA) are common events that have been found to occur in human ageing and in patients with mitochondrial myopathies. The mechanisms by which these deletions occur remain unclear, but several mechanisms have been proposed, such as slipped-mispairing, illegitimate recombination, and oxidative reactions elicited by free radicals. In addition, the DNA to...

The phenomenological model of Khossravi and Connors (1992) has been adopted to calculate the electrophoretic mobility of drugs at different concentrations of solvents in a binary mixture. The accuracy and predictability of the model have been evaluated employing 14 experimental data sets by using average percentage mean deviation (APMD). The obtained APMD for correlative and predictive studies ...

In the present study, an alternative procedure for two-dimensional (2D) electrophoretic analysis in proteomic investigation of the most represented basic muscle water-soluble proteins is suggested. Our method consists of Acetic acid-Urea-Triton polyacrylamide gel (AUT-PAGE) analysis in the first dimension and standard sodium dodecyl sulphate polyacrylamide gel (SDS-PAGE) in the second dimension...

In this paper, we present the pattern analysis of autoradiogram images obtained in two-dimensional gel electrophoresis based on the RLGS (restriction landmark genome scanning) method [1]. On a RLGS autoradiogram image, several thousands of spots can be observed; one spot corresponds to one genetic locus and its intensity re ects the copy number of the restriction landmark. In the rst step of th...

In this review we examine the current state of analytical methods in proteomics. The conventional methodology using two-dimensional electrophoresis gels and mass spectrometry is discussed, with particular reference to the advantages and shortcomings thereof. Two recently published methods which offer an alternative approach are presented and discussed, with emphasis on how they can provide info...

PURPOSE To investigate changes in protein profiles of posterior sclera in guinea pigs during development of form deprivation myopia and recovery. METHODS Three groups of guinea pigs (developing form deprivation myopia, recovering from the myopia and normal control) were evaluated for protein profiles of the posterior sclera using two-dimensional gel electrophoresis. Protein spots with a diffe...

Analysis of images obtained from two-dimensional gel electrophoresis (2D-GE) is a topic of utmost importance in bioinformatics research, since commercial and academic software available currently has proven to be neither completely effective nor fully automatic, often requiring manual revision and refinement of computer generated matches. In this work, we present an effective technique for the ...

Polyadenylylated mRNA from etiolated pea stem segments treated with or without 20 muM indoleacetic acid (IAA) for various periods of time was assayed by translating it in a wheat germ extract containing [(35)S]methionine and separating the translation products by two-dimensional gel electrophoresis. Within 2 hr IAA causes at least five mRNA sequences to increase in translational activity, relat...