Knowledge of transcriptional regulatory interactions (TRIs) is essential for exploring functional genomics and systems biology in any organism. While several results from genome-wide analysis of transcriptional regulatory networks are available, they are limited to model organisms such as yeast ( 1 ) and worm ( 2 ). Beyond these networks, experiments on TRIs study only individual genes and prot...

Blood paper cards provide an effective DNA storage method. In this study, we used three DNA dissolving reagents (Tris-EDTA [TE] buffer, Tris-HCl buffer, and water) and one common commercially available kit (DN131 from MRC Inc) to elute DNA from 105 human blood paper cards collected up to 10 years ago. These DNA samples were used as templates for amplification of a single nucleotide polymorphism...

The exploitation of intercalation techniques in the field of two-dimensional layered materials offers unique opportunities for controlling chemical reactions in confined spaces and developing nanocomposites with desired functionality. In this study, the exploitation of the novel and facile "one-pot" anion-exchange method for the functionalization of layered double hydroxides (LDHs) is demonstra...

Starting from tris(trimethylsilyl)silane the novel 2-chloro-3,4-bis-tris(trimethylsilyl)silyl-cyclo-diphospha-diazenium-tetrachloridogallate salt (7) was prepared in a six step synthetic procedure including the synthesis of N-tris(trimethylsilyl)silyl-aminodichlorophosphine (4) and 1,3-dichloro-2,4-bis-tris(trimethylsilyl)silyl-cyclo-diphospha-diazane (5). All intermediate products along the re...

PURPOSE To optimize direct magnetic resonance (MR) arthrography by determining the effect of dilution of gadolinium in iodinated contrast, saline, or albumin on T1-weighted, T2-weighted, and gradient-recalled echo (GRE) images, and the effect of scanner field strength. MATERIALS AND METHODS Gadopentetate dimeglumine was diluted into normal saline, albumin, or iodinated contrast (0.625 mmol/li...

A tris-borate buffer solution of = 0.1 and pH 8.9 is shown to be satisfactory for use in the elect-rophoresis of human and bovine sera by the moving-boundary technic. Comparison of the patterns obtained by the use of tris-borate and barbital buffer is presented. Similar numbers of electrophoretically distinct components are found by the use of either buffer. Although the calculated mobility of ...

Two new non-barbiturate buffers have been formulated for serum protein electrophoresis on cellulose acetate membranes. Both buffers give five distrinct fractions and are suitable for all systems, but have been primarily designed to meet the needs of the Gelman "Sepratek" system. One buffer is a tris(hydroxymethyl)aminomethane (Tris)/hippurate formulation; the other is composed of Tris/N-[tris(h...

Background Cardiac magnetic resonance (CMR) late gadolinium enhancement (LGE) and early gadolinium enhancement (EGE) are powerful diagnostic tools for acute cardiomyopathies. LGE is associated with impaired prognosis in ischemic and in chronic non-ischemic cardiomyopathy (NICM), but the impact of gadolinium enhanced CMR on prognosis in recent onset NICM is less well established. We performed a ...