Platinum (Pt)-based antitumor agents have been the mainstay of cancer chemotherapy for the last three decades. While multiple mechanisms are responsible for treatment failure, deficiency in drug transport is an important contributor. The human high-affinity copper (Cu) transporter-1 (hCtr1) can also transport Pt-based drugs including cisplatin (cDDP) and carboplatin. Reduced hCtr1 expression fr...

We read with great interest the article in mBio regarding the discovery of a third colistin resistance determinant, mcr-3, in plasmid pWJ1 isolated from porcine Escherichia coli in China (1). This discovery and the subsequently reported discovery of a fourth colistin resistance determinant, mcr-4 (2), provide further support for our hypothesis that the mcr determinants (phosphoethanolamine tran...

Antibiotic therapy can affect not only the pathogenic bacteria, but also commensal microorganisms in the gut flora of humans, which might serve as a reservoir of antimicrobial resistance genes. Escherichia coli can be found widely in nature, and can also be implicated in human infectious diseases. Therefore, fecal E. coli is often considered as a good indicator for selection pressure imposed by...

Most patients with diabetes die from cardiac or arterial disease, for which there are limited therapeutic options. Free Cu(2+) ions are strongly pro-oxidant, and chelatable-Cu(II) is increased in the diabetic heart. We reported previously that treatment by Cu(II)-selective chelation with triethylenetetramine (TETA) evokes elevated urinary Cu(II) in diabetic rats and humans in whom it also impro...

Salmonella is one of the most common causes of food poisoning in the European Union and the United States of America. Free-living birds are known as a reservoir for the different serovars of Salmonella, including S. Typhimurium, S. Enteritidis, S. Infantis, S. Newport and S. Hadar, which may play an important role in the epidemiology of salmonellosis in farm animals, particularly poultry. Also,...

OBJECTIVE In-111 ((111)In)-labeled octreotide has been clinically used for imaging somatostatin receptor-positive tumors, and radiolabeled octreotide analogs for positron emission tomography (PET) have been developed. Cu-64 ((64)Cu; half-life, 12.7 h) is an attractive radionuclide for PET imaging and is produced with high specific activity using a small biomedical cyclotron. The aim of this stu...

A total of 310 Salmonella isolates were isolated from 6 broiler farms in Eastern China, serotyped according to the Kauffmann-White classification. All isolates were examined for susceptibility to 17 commonly used antimicrobial agents, representative isolates were examined for resistance genes and class I integrons using PCR technology. Clonality was determined by pulsed-field gel electrophoresi...

Received 16 June 2003 Revised 15 August 2003 Accepted 18 August 2003 The complete 64508 bp nucleotide sequence of the IncP-1b antibiotic-resistance plasmid pB10, which was isolated from a waste-water treatment plant in Germany and mediates resistance against the antimicrobial agents amoxicillin, streptomycin, sulfonamides and tetracycline and against mercury ions, was determined and analysed. A...

The aims of the current study were to detect the virulence factors and antibiotic resistance of Shiga toxin-producing E. coli, in animal milk and dairy products in Iran. After E. coli dentification with culture method, PCR assay were developed for detection of pathogenic genes, serotypes and antibiotic resistance genes of E. coli. Results showed that out of 719 samples, 102 (14.18%) were confir...

Divalent metal transporter-1 (DMT1) is a divalent cation transporter that plays a key role in iron metabolism by mediating ferrous iron uptake across the small intestine. We have previously identified several small molecule inhibitors of iron uptake (4). Using a cell line that stably overexpresses DMT1, we screened the ability of these inhibitors to specifically block this transporter's activit...