Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene expression was studied in differentiating brown adipocytes. Northern blot analysis showed that GAPDH mRNA levels increased during differentiation of precursor cells into mature adipocytes, mainly in the initial stages of the differentiation process. Insulin, tri-iodothyronine (T(3)) and norepinephrine, the main regulators of brown adipose ti...

The design of bioactive scaffolds with improved mechanical and biological properties is an important topic of research. This paper investigates the use of polymer-ceramic composite scaffolds for bone tissue engineering. Different ceramic materials (hydroxyapatite (HA) and β-tri-calcium phosphate (TCP)) were mixed with poly-ε-caprolactone (PCL). Scaffolds with different material compositions wer...

Certain organic phosphorus esters produce sensorimotor axonopathy in man and other species. There is an excellent correlation between the capacity of an organophosphorus compound to produce axonopathy and its ability to inhibit brain neurotoxic esterase (NTE) in hens. Because NTE is present in peripheral lymphocytes of both hen and man, it has been suggested that the lymphocyte enzyme might be ...

Tri-ortho-cresyl phosphate (T.O.C.P.) is an organic phosphorus compound commonly used in industry as a plasticizer, and is so used in the manufacture of lacquers, dopes, synthetic fabrics, and synthetic lignin resin; it is used as a water-proofing agent in textile manufacture. It is said to be an excellent grinding medium for pigments; it is also used in making polyvinyl chloride sheeting and f...

Hydroxylamine reacts as an oxygen nucleophile, most likely via its ammonia oxide tautomer, towards both phosphate di- and triesters of 2-hydroxypyridine. But the reactions are very different. The product of the two-step reaction with the triester TPP is trapped by the NH2OH present in solution to generate diimide, identified from its expected disproportionation and trapping products. The reacti...

In a previous communication (Heald, 1957 b) it was shown that the 'phosphoprotein' fraction of cerebral tissues was implicated in the rapid phosphateexchange reactions promoted by applied electrical pulses (Heald, 1956b). It was pointed out that owing to the crudeness of the analytical methods employed the nature of the phosphoprotein could not be determined, more especially since its estimatio...

2. Swine pyloric juice also exhibited two proteolytic pH maxima, at pH values 1-6 and 2-6, which are lower than those of swine or human fundic extracts or of human gastric juice. 3. Crystalline swine pepsin can be separated into two components, one of which digested proteins with pH maxima close to those found with swine fundic mucosa and the other with maxima close to those found with pyloric ...