BACKGROUND Human cholinesterases can be used as a bioscavenger of organophosphate toxins used as pesticides and chemical warfare nerve agents. The practicality of this approach depends on the availability of the human enzymes, but because of inherent supply and regulatory constraints, a suitable production system is yet to be identified. RESULTS As a promising alternative, we report the creat...

Abiotic stresses create an unfavourable environment for plant growth, increasing the possibilities of low yield and associated economic loss. Several steps have been taken to address this problem. During last twenty years, techniques genetic engineering/transgenic breeding made significant advances in gene manipulation inciting desirable traits transgenic plants. Transgenic allow us identify po...

Aim. The aim of the study was obtaining Camelina sativa (L.) Crantz lines with yeast genes trehalose synthesis TPS1 and TPS2 to increase their resistance drought. Methods. Seeds C. genotype FEORZhYaF-1 were used for in vitro culture establishment. For this hypocotyl segments shoot meristems 5-days-old camelina seedlings cultivated on three different nutrient media regeneration supplemented vari...

Finger millet is susceptible to abiotic stresses, especially drought and salinity stress, in the field during seed germination and early stages of seedling development. Therefore developing stress tolerant finger millet plants combating drought, salinity and associated oxidative stress in these two growth stages is important. Cellular protection through osmotic adjustment and efficient free rad...

Silicon (Si) is one of the most prevalent elements in the soil. It is beneficial for plant growth and development, and it contributes to plant defense against different stresses. The Lsi1 gene encodes a Si transporter that was identified in a mutant Japonica rice variety. This gene was not identified in fourteen Malaysian rice varieties during screening. Then, a mutant version of Lsi1 was subst...

The use of transgenic plants for the expression of therapeutic recombinant proteins is gradually gaining acceptance in the scientific community (Moffat, 1995). There are many attractions to this technology, including the possibility of manufacture on an agricultural scale for bulk production and to reduce costs, as well as the range of plant hosts available and the stability of transgenic lines...

plant quality in herbivores’ diet may affect the performance of both herbivore and its parasitoids. in the present research, parasitism capacity of diadegma semiclausum (hellen) on plutella xylostella (l.) reared on different genetically manipulated brassica plants including the canola’s progenitor (brassica rapa l.), two cultivated canola cultivars (cultivar-opera and cultivar-rgs003), one hyb...

Receptor-like kinases (RLK) play fundamental roles in plant growth and stress responses. Compared with other RLKs, little information is provided concerning the S-locus LecRLK subfamily, which is characterized by an extracellular G-type lectin domain and an S-locus-glycop domain. Until now, the function of the G-type lectin domain is still unknown. In a previous research, we identified a Glycin...

The acetoacetyl-CoA reductase gene (phbB) of Ralstonia eutropha and the poly[(R)-(-)-3-hydroxyalkanoate] synthase gene (phaCAC) of Aeromonas caviae were introduced into tobacco plant by Agrobacterium mediated transformation method. The resulting transgenic tobacco expressed both introduced genes and the expression of these genes was confirmed by enzymatic analysis and western blotting. GC-MS an...

Background: In consideration for the increasing widespread use of genetically modified (GM) crops, one of the important issues for assessment is the effect of GM crops on soil microbial communities Objectives: In this study, T2 chitinase-transgenic cotton (line #57) and its non-transgenic line were investigated for bacterial and fungal dynamics...