Background: Electroporation is a valuable tool for small interfering RNA (siRNA) delivery into cells because it efficiently transforms a wide variety of cell types. Since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect MDA-MB 468 human breast cancer cell with siRNA. ...

background: tuberculosis (tb) remains as a major cause of death around the world. construction of a new vaccine against tuberculosis is an effective way to control it. several vaccines against this disease have been developed. the aim of the present study was to cloning of tb10.4 gene in pcdna3.1+ plasmid and evaluation of its expression in eukaryotic cells. methods: firstly, tb10.4 fragment wa...

A variety of methods have been described for introducing DNA into cultured mammalian cells. Among these, calcium phosphate (CaPO4)-mediated transfection has gained wide acceptance due to the procedure's low cost, simplicity and suitability for a wide range of cell types. However, this method is limited by relatively low transfection efficiencies and poor reproducibility. To improve the utility ...

The introduction of nucleic acids into cells is one of the most valuable and frequently used tools of biological science. Transfection methods are used for a range of applications, including gene function studies, modulation of gene expression, biochemical mapping, mutational analysis and production of recombinant proteins. The transfection techniques which are commonly used today can be classi...

The in vivo activitty of the recBC nuclease was assayed by transfection of isogenic rec+ and rec-spheroplasts with bacteriophage DNA of various origin and structure. The results indicate that the recBC nuclease can limit transfection at several stages during the production of an infective center; such limitations depend primarily on whether the DNA is in, or assumes, a nuclease-sensitive struct...

Introduction The transfection of nucleic acids into cells is crucial for the study of many aspects of neuronal cell biology. These include investigating gene and protein function by knocking down target proteins via RNA interference (RNAi) or microRNAs, expressing tagged proteins to track their subcellular localization, behavior, and turnover; and expressing mutant versions of proteins to study...

OBJECTIVE The introduction of recombinant genes into endothelial cells provides a method to study specific gene products and their effect on cell function. In addition, endothelial cells can be used for implantation into vessels or prosthetic vascular grafts. Because transfection efficiencies in human endothelial cells have been low, it is important to develop improved gene transfer techniques....

Purification and isolation of cellular target proteins for monoclonal antibody (MAb) production is a difficult and time-consuming process. Immunization of mice with murine cell lines stably transfected with genes coding for xenogenic target molecules is an alternative method for mouse immunization and MAb production. Here we present data on transfection efficiency of some commercial reagents us...