Digitized growth pattern images of wood-rotting fungi Fomitopsis palustris (FOM) and Trametes versicolor (TRA) were used with a video processing program new formula to calculate the numerical results microbial inhibition by an active substance (wood vinegar). First, daily collected generate calibration curves for each fungus. FOM stopped growing in 7 days, whereas TRA 11 days. The showed R2 = 0...

Pharmaceutical products (PPs) are considered as emerging micropollutans in wastewaters, river and seawaters, sediments. The biodegradation of PPs, such ciprofloxacin, amoxicillin, sulfamethoxazole, tetracycline by enzymes aqueous solution was investigated. Laccase from Trametes versicolor immobilized on silica monoliths with hierarchical meso-/macropores. Different methods enzyme immobilization...

Mycelium-bonded bio-composites are promising new materials for replacing non-sustainable products. In such composite systems, fungal mycelia work as an adhesive, bonding together lignocellulosic substrate particles. this work, we focus on the two groups of mycelium-bonded bio-composites: as-grown foams and hot-pressed (densified) panels. We used Trametes versicolor mycelium yellow birch wood pa...

The upstream sequences of all published lignin peroxidase and manganese peroxidase genomic clones from Phanerochaete chrysosporium were analyzed. This analysis revealed the presence of putative activator protein-2 (AP-2) recognition sequences in 11 of 15 lignin peroxidase genes. The lignin peroxidase clone GLG6 and the manganese peroxidase gene (mnp-1) have two copies of putative AP-2 sequence ...

The oxidation of polycyclic aromatic compounds was studied in systems consisting of laccase from Trametes versicolor and so-called mediator compounds. The enzymatic oxidation of acenaphthene, acenaphthylene, anthracene, and fluorene was mediated by various laccase substrates (phenols and aromatic amines) or compounds produced and secreted by white rot fungi. The best natural mediators, such as ...

Laccases are multicopper oxidoreductases that can be used in biofuel cells to improve cathode performance by cathodic oxygen reduction. Here we present a laccase from the ligninolytic white-rot fungus Pycnoporus sanguineus that, in contrast to the Trametes versicolor laccase, can be produced in the absence of inducers in a standard culture medium. After 7days of cultivation the activity of this...

Melanin formation and assembly by fungi has largely been investigated mainly for its importance in pathogenesis, as well as to establish the functions and biosynthetic pathways of melanin formed during the process of successional wood decay. It is known that melanin formation varies based on fungal species, especially melanin produced by ascomycetes versus basidiomycetes, and that the mechanism...

The potential of crude enzyme extracts, obtained from solid state cultivation of four white-rot fungi (Trametes versicolor, Bjerkandera adusta, Ganoderma applanatum and Phlebia rufa), was exploited to modify wheat straw cell wall. At different fermentation times, manganese-dependent peroxidase (MnP), lignin peroxidase (LiP), laccase, carboxymethylcellulase (CMCase), avicelase, xylanase and feru...

In the presence of substrates such as Remazol Blue and 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonate) (ABTS), laccases Coriolus (Trametes) versicolor can also oxidize non-phenolic lignin model compounds. Veratryl alcohol (I) and 1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)-propane-1,3-diol (III) were oxidized by laccase and mediator to give the alpha-carbonyl derivatives. The beta-1 lignin m...

Agricultural wastewater is a major source of herbicides, which pose environmental and health concerns owing to their substantial use poor elimination rate in conventional treatment plants. White-rot fungi are versatile degrading xenobiotics; however, the key problem encountered with application actual scenarios competition indigenous microorganisms, mainly bacteria. To address this barrier, two...