Brucellosis is considered the major zoonosis in developing countries. In susceptible ani‐ mal species, diagnosis of brucellosis remains a challenge due to the variety of clinical signs that it shares with a wide range of diseases. At present, isolation of Brucella is con‐ sidered the gold standard for diagnosis of brucellosis; because of its low sensitivity and be‐ coming potentially hazardous ...

Traditional methods for detection and serotyping of enterovirus infections are virus isolation and immunofluorescence assay (VI-IFA), which are labor-intensive and time-consuming. Recently, VP1 gene has been targeted to develop a CODEHOP-based RT-PCR (VP1-CODEHOP) for the same purpose. In this study, we conducted a 5-year enterovirus surveillance comparing the VI-IFA and VP1-CODEHOP tests. Thro...

Ocular toxoplasmosis is considered to be the most commonly recognized cause of chorioretinitis in the United States. It is commonly believed that the majority of cases of acute toxoplasmic chorioretinitis involving adults in the United States are late sequelae of congenital infection and that the condition is rarely associated with acute postnatally acquired infection. We report here the clinic...

BACKGROUND Serological methods to monitor Pseudomonas aeruginosa colonisation in patients with cystic fibrosis (CF) are advocated but the diagnostic value of a commercially available P aeruginosa antibody test to detect early and chronic P aeruginosa colonisation in a non-research setting has not been assessed. METHODS Colonisation with P aeruginosa was estimated by regular culture of sputum ...

The first serological test for syphilis, developed by Wassermann, Neisser, and Bruck (1906), was a complement-fixation test for reagin activity. Many tests for reagin activity have been developed since then, including the Kline, Kahn, Hinton, Kolmer, Mazzini, and VDRL slide tests. However, none of these is absolutely specific for syphilis. The VDRL slide test (Harris, Rosenberg, and Riedel, 194...

INTRODUCTION We tested a commercially available rapid hepatitis C virus (HCV) test assay for its potential use for analyses of corpses as a screening option for index persons who have died after mass-casualty incidents in high-prevalence settings in the field. MATERIALS AND METHODS 50 blood samples were drawn from 16 recently deceased confirmed HCV-positive patients whose corpses were stored ...