Barnacle cement is an underwater adhesive insoluble protein complex. Marine proteins secreted by the invertebrates such as barnacles and mussels have potential application as powerful adhesives as they insolubilize and adhere to variety of substrates in aqueous environment. The adhesive properties of the barnacle adhesive proteins have been utilized for various dental and medical purposes. Thes...

The toxicity of amyloid-forming proteins is correlated with their interactions with cell membranes. Binding events between amyloidogenic proteins and membranes result in mutually disruptive structural perturbations, which are associated with toxicity. Membrane surfaces promote the conversion of amyloid-forming proteins into toxic aggregates, and amyloidogenic proteins, in turn, compromise the s...

The combination of cell-penetrating poly(disulfide)s with biotin-streptavidin biotechnology provides a simple, general, non-toxic method that avoids significant endosomal capture and delivers proteins directly, even to the nucleoli.

UNLABELLED To survive environmental challenges, biological systems rely on proteins that were selected by evolution to function in particular cellular and conditional settings. With the advent of protein design and synthetic biology, it is now possible to construct novel proteins that are not biased by eons of selection in natural hosts. The availability of these sequences prompts us to ask whe...

BACKGROUND Recombinant expression of toxic proteins remains a challenging problem. One potential method to shield toxicity and thus improve expression of these proteins is to encapsulate them within protein compartments to sequester them away from their targets. Many bacteria naturally produce so-called bacterial microcompartments (BMCs) in which enzymes comprising a biosynthetic pathway are en...

Various neurodegenerative diseases are characterized by the accumulation of amyloidogenic proteins such as tau, α-synuclein, and amyloid-β. Prior to the formation of these stable aggregates, intermediate species of the respective proteins-oligomers-appear. Recently acquired data have shown that oligomers may be the most toxic and pathologically significant to neurodegenerative diseases such as ...

Two mutant strains of Escherichia coli BL21(DE3), called C41(DE3) and C43(DE3) and originally described by Miroux and Walker, are frequently used to overcome the toxicity associated with overexpressing recombinant proteins using the bacteriophage T7 RNA polymerase expression system. Even when the toxicity of the plasmids is so high that it prevents transformation in the strain BL21(DE3), the to...

Initially identified in pathogenic Gram-negative bacteria, the two-partner secretion (TPS) pathway, also known as Type Vb secretion, mediates the translocation across the outer membrane of large effector proteins involved in interactions between these pathogens and their hosts. More recently, distinct TPS systems have been shown to secrete toxic effector domains that participate in inter-bacter...

Synthesis of long peptides is generally considered as a challenge to peptide chemists, in addition producing significant amounts toxic waste, such DMF. Here we show that using solvent-less methods, ball milling, enabled the production hexapeptide Boc-(Ala-Phe-Gly)2-OBn at gram scale with high overall yield (77 %, 5 linear steps). This longest chain synthesized mill date, which amino acid sequen...

Copper (Cu) is a trace element necessary for the growth and development of all living organisms, third most abundant metal in body after iron zinc. essential maintaining life processes cells, because several copper-dependent enzymes play an important role key physiological like cellular respiration, oxygen radical scavenging, transport neurotransmitter synthesis. Maintaining copper homeostasis ...