BACKGROUND alpha,alpha-Trehalase, located on renal proximal tubules, is a glycoprotein that hydrolyses alpha,alpha-trehalose to two glucose molecules. Urinary trehalase reflects damage to renal proximal tubules, but its activity has not been measured routinely because measurement of catalytic activity is rather complicated and because conventional assays for enzyme activity might not reflect al...

Recombinant protein production in Pichia pastoris is based on alcohol oxidase promoterswhich are regulated by methanol. However, the use of methanol has several disadvantages,which is why current trends in bioprocess development with Pichia pastoris (P. pastoris) arefocusing on methanol mixed feeding strategies. This work aimed to develop a new experimentalmethod and compare the effect of vario...

PURPOSE Pre-eclampsia (PE) is associated with intravascular inflammation and endothelial dysfunction. Interestingly, endocan plays a predominant role in the vascular inflammation and is considered as a biomarker of endothelial dysfunction. The aim of this study was to explore whether the endocan levels in serum and placenta were different between pregnant women with PE and the normal pregnancie...

this study was conducted in acompletely randomized block design with three replications using three concentrations of 107, 105, and 103cfu/ml of azotobacter isolated from the rhizosphere of karun varieties of barley (az. salentris) and standard az.chrococom along with control (zero concentration of bacteria). the aim of this study was to study the reactions of barley plantin response to inocula...

Background: Although a simple and direct method does not exist for the detection of chlamydial infections, there are situations in which reliable serological tests, with sensi-tivity related to a specific antigen, could be helpful. Objective: The aim of this study was to clone the first 1100 bp of the C. trachomatis outer membrane protein 2 (omp2) gene in order to prepare a recombinant protein ...

An indirect ELISA incorporating a protein A-peroxidase conjugate was developed for detecting antibodies to swine vesicular disease virus (SVDV) in pig sera. This test and a conventional virus neutralization test were found to be equally sensitive. A total of 2846 pig sera collected from various abattoirs in South Africa were tested using the indirect ELISA. No serological evidence of infection ...

A systematic study on the influence of pigments and sample aging on casein identification was performed on 30 reconstructed paints. The protein in all the paints was extracted into solution for analysis. The amount of protein that can be retrieved for solution-based analysis in each of the reconstructed paints was studied with a well-developed NanoOrange method before and after artificial aging...

Background: The witches’ broom disease of lime caused by <span style="font-variant: normal; font-style: normal; font-family: Capitolium...

&#x0D; The recombinant antibodies RB690, RB691, RB692 and RB693 detect by ELISA a synthetic peptide from the Drosophila melanogaster Nop56 protein.

BACKGROUND: Many additives have been recommended to calf feed but the effects of each supplement have been under investigation especially during weaning stress. OBJECTIVES: The effects of dietary supplementation of probiotic (Protexin) and chromium-methionine che- late (Cr-Met) on triiodothyronine (T3), thyroxine (T4), total protein, albumin, zinc, and gr...