In this study, we established a rapid and sensitive method for the detection of viable Salmonella Typhimurium, Staphylococcus aureus, Listeria monocytogenes in milk using biotin-exposure-based immunomagnetic separation (IMS) combined with sodium dodecyl sulfate (SDS), propidium monoazide (PMA), multiplex real-time PCR (mRT-PCR). We used IMS to lessen assay time isolation target bacteria. then o...

In ${\mathrm{Ta/(Co,Fe)B/HfO}}_{2}$ stacks, a gate voltage drives, in nonvolatile way, the system from an underoxidized state exhibiting in-plane anisotropy (IPA) to optimum oxidation level resulting perpendicular (PMA) and further into overoxidized with IPA. The $\mathrm{IPA}\phantom{\rule{0.1em}{0ex}}\ensuremath{\rightarrow}\phantom{\rule{0.1em}{0ex}}\mathrm{PMA}$ regime is found be significa...

In this study, we demonstrate that phorbol 12-myristate 13-acetate (PMA)-activated protein kinase C (PKC) induced migration in A172 glioblastoma cells via Src. PMA treatment induced tyrosine phosphorylation of Crk-associated substrate (Cas) and formation of a complex with Crk, followed by Rac1 activation, a downstream effector of Cas/Crk complex. These effects were blocked by a tyrosine kinase ...

A role for protein kinase C in arachidonate mobilization was demonstrated. Treatment of rat platelets with phorbol myristate acetate (PMA) or the diacylglycerol 1-oleoyl-2-acetylglycerol increased the transfer rate of arachidonate (AA) from phosphatidylcholine to phosphatidylethanolamine and stimulated AA release. The transfer dose-dependently induced by PMA was inhibited by staurosporine. Ethe...

A B S T R A C T Human neutrophils contain receptors for phorbol myristate acetate (PMA), a complex lipid that induces them to generate superoxide (02). Binding of PMA to these receptors displays specificity, reversibility, and high affinity. The receptor's apparent KD was -0.29 nM and multiple copies (-2.1±0.6 x 105) were present per neutrophil. We found that the timing and magnitude of the neu...

Regulation of eotaxin expression was investigated in U-937 cells, a human monocyte-like cell line. Eotaxin mRNA was induced by tumor necrosis factor-α (TNF-α; 0.1-100 ng/ml) and phorbol 12-myristate 13-acetate (PMA; 0.01-1 μM). PMA-induced eotaxin mRNA expression was of greater magnitude and was maximal at a later time point than TNF-α-induced expression (16 h vs. 2 h after stimulation), which ...

Hypoxia impairs metabolic functions by decreasing activity and expression of ATP-consuming processes. To separate hypoxia from systemic effects, we tested whether hypoxia at high altitude affects basal and PMA-stimulated leukocyte metabolism and how this compares to acute (15 min) and 24 h of in vitro hypoxia. Leukocytes were prepared at low altitude and ∼24 h after arrival at 4559 m. Mitochond...

In human platelets, phorbol esters, such as phorbol-12-myristate-13-acetate (PMA), induce morphological changes, including pseudopod formation and the swelling and fusion of intracellular granule membranes with those of the surface-connected canalicular system, effects which have been attributed to activation of protein kinase C. However, a novel intracellular histamine antagonist, N,N-diethyl-...

The transcriptional regulation of the fibronectin (FN) gene in hepatoma cells by phorbol myristate acetate (PMA) was investigated. PMA increased the synthesis and mRNA levels of FN and its promoter activity in Hep3B hepatoma cells. The PMA-induced activation of FN expression was blocked by a protein kinase C (PKC) inhibitor and did not require a new protein synthesis. Deletion analysis revealed...