The purpose of this study is to establish a gene delivery system for interstitial tissue-specific protein expression in mice testes using modified recombinant baculovirus. Green fluorescent protein (GFP)-expressing recombinant baculovirus (GFP-baculovirus), in which the insect cell-specific polyhedron promoter was replaced by the cytomegalovirus (CMV)-IE promoter, was used to transfect testicul...

To optimize the procedure for testis tissue xenografting, we grafted 2, 4, 8, or 16 small fragments of immature porcine testis tissue under the back skin of immunodeficient castrated mice (n = 10 mice/group). At 8 months post grafting, the graft recovery rate did not differ between groups; however, not only the total but also the average graft weights were higher (by approximately 12-fold and a...

Background Our recent findings have shown that the rat testis is subjected to significant oxidative stress during the early phase of diabetes induced by Streptozotocin (STZ). In the present study, we have investigated whether oral supplementation of β-carotene (BC) to pubertal rats would provide protection against diabetes associated oxidative stress in testis and liver. MaterialsAndMethods Mal...

Here, we describe a novel member in the group of membrane-anchored chymotrypsin (S1)-like serine proteases, namely testis serine protease 1 (T-SP1), as it is principally expressed in testis tissue. The human T-SP1 gene encompasses 28.7 kb on the short arm of chromosome 8 and consists of seven exons. Rapid amplification of cDNA ends (RACE) experiments revealed that due to alternative splicing th...

background: irreversible electroporation (ire) is a novel tumor ablation technique. ire is associated with high electrical fields and is often reported in conjunction with thermal damage caused by joule heating. for good response to surgery it is crucial to produce minimum thermal damage in both tumoral and healthy tissues named non-thermal irreversible electroporation(ntire). non-thermal irrev...

Abstract Introduction With the delay of reproductive age, disorders related to advanced age have gradually attracted widespread attention. Studies shown that decline in male fertility due aging is manifested as a decrease semen parameters and spermatogenetic cells histologically. O-GlcNAc (O-acetylglucosamine) glycosylation post-translational modification exists widely cells. The level UDP-GlcN...

The purpose of this review is to describe how the immune cells present in the testis interact with the germinal epithelium contributing to survival or apoptosis of germ cells (GCs). Physiologically, the immunosuppressor testicular microenvironment protects GCs from immune attack, whereas in inflammatory conditions, tolerance is disrupted and immune cells and their mediators respond to GC self a...