Abstract Air-liquid interface (ALI) cultures are commonly used for 3D modelling of the human airways and to examine effects direct exposure airborne toxicants. We explored suitability epithelial cell lines (RPMI 2560, HBEC-3KT, CALU-3 A549) primary cells (nasal, bronchiolar small airways) grown at ALI as a physiologically relevant model whether environmental exposures can increase susceptibilit...

BACKGROUND Herb-partitioned moxibustion (HPM) at Tianshu (ST25) and Qihai (RN6) has been used to treat Crohn's disease (CD). Injury to intestinal epithelial tight junctions (TJs) is the leading cause of CD onset with under expression of TJ-related proteins such as occludin, claudin-1, and zonula occludens protein-1 (ZO-1). This study aimed to investigate whether HPM can change the permeability ...

Abstract Background As tricuspid transcatheter edge-to-edge repair (TEER) gains momentum, a better understanding of coaptation gap size and location becomes increasingly important to properly select patients for this therapy. The valve is complex patient phenotypes may be highly variable. It currently unknown how the impact procedural strategy clinical outcomes. Purpose To characterize in recei...

Introduction: Inflammatory bowel disease (IBD) is a chronic relapsing and remitting with rising incidence globally. Circulating exosomes play great roles in IBD pathogenesis through exosomal cargoes, especially impacting the function of endothelial barriers. Transendothelial electrical resistance (TEER) measurement widely used non-invasive label-free strategy to monitor barrier vitro . This stu...

Blood brain-barrier (BBB) in vitro models have been widely reported in studies of the BBB phenotype. However, established co-culture systems involve brain endothelial cells, astrocytes, neurons and pericytes, and therefore are often consuming and technically challenging. Here we use mesenchymal system cells (MSC) as a potential substitute for pericytes in a BBB model. Both MSC and pericyte mark...

Orthosiphon stamineus Benth. (Lamiaceae) is commonly used for the treatment of kidney diseases, but its role in intestinal barrier function remains unknown. The present study investigated protective effects O. extract (OE) against oxidative stress-induced injury to small epithelium and possible mechanism. High-performance liquid chromatography fingerprinting was analyze OE. Oxidative stress ind...

Sensitive skin is a common condition that can severely impact quality of life. Several mechanisms are thought to be involved, including those affecting the barrier function, hydration and innervation. Our Goal was investigate clinically on in vitro models, benefit formulations dedicated sensitive containing Aquaphilus dolomiae extract-G3 (ADE-G3) function hydration, as well protective responses...

Blood Brain barrier (BBB) is a natural protective wall in the brain to restrict invasion of xenobiotics or toxic chemicals. This, turn, becomes major obstacle for researchers and industry people formulating new drugs treat disorders like tumors, Alzheimer's disease, multiple sclerosis, meningitis, so on. The purpose this research study in-vitro cytotoxicity & BBB permeation curcumin-loaded ...