نتایج جستجو برای: technique of repeat
تعداد نتایج: 21201298 فیلتر نتایج به سال:
To resect a small liver tumor located in the paracaval portion, we adopted transhepatic enucleation, i.e. enucleation of the tumor from the transected plane along the main portal fissure. In contrast to caudate lobectomy, this procedure can save dissection around the liver and vena cava, which would increase operation time and blood loss especially in repeat surgery after removal of Couinaud's ...
myotonic dystrophy (dm), one of the most common forms of muscular dystrophy, is an inherited disorder of the muscles and other body systems. dm is a progressive genetic disorder with a triplet repeat autosomal mutation that affects an estimated 1 in 8000 people. myotonic dystrophy type 1, also known as steinerts disease, and type 2 caused by mutation in dmpk and cnbp genes, respectively. in eac...
use of random amplified polymorphic dna to identify candida species, originated from cancer patients
introduction: recently, the incidence of fungal infections dramatically increased with an appearance of many novel species due to different criteria. therefore, several molecular methods have been established for identification of these agents which cause human and animal disorder based on genomic dna. among these methods, rapd-pcr technique is a powerful discriminative method based on amplifie...
Polymorphic dinucleotide repeat loci ('microsatellite markers') are found in varying abundance throughout the genomes of most organisms. They have been extensively used for genetic studies, but conventional techniques used for their genotyping require sophisticated equipment. Microplate array diagonal gel electrophoresis (MADGE) has previously been extended to economical high-throughput genotyp...
DM1 is caused by CTG repeat expansion in the 3'-UTR of the DMPK gene. DM1 patients have expansions of greater than 50 repeats and up to many thousands. The intention of the present study is the establishment of reliable and rapid polymerase chain reaction methodology in early screening of DM1 patients and their family members. PCR followed by TP-PCR was assessed for screening of 27 cases (from ...
objective(s) varicocele is associated with impaired testicular function and male infertility, but the molecular mechanisms by which fertility is affected have not been satisfactorily explained. the aim of our study was to investigate whether or not the polymerase gamma (polg) polymorphism is associated with iranian varicocele patients. materials and methods we determined the polg cag repeat ...
mutations of the androgen receptor (ar) gene give rise to a wide array of phenotypic abnormalities. various mutations of the ar gene and expanded polyglutamine repeats (cag) at exon 1 of the gene have been reported in patients with infertility and neurodegenerative diseases. however, the role of the ar gene trinucleotides repeats has not been systemically studied in those with hypospadias or ge...
herpes simplex virus 1 (hsv-1) unspliced 8.3 latency associated transcript (lat), which located in the long repeat sequences, has been shown to contain at least 16 open reading frames (orf: a-p). one of these orf, orf p, maps almost entirely antisense to hsv-1 neurovirulence gene, icp34.5. both orf p and icp34.5 are located in the long repeat and are antisense overlapping genes. therefore, in o...
PURPOSE Cross-contamination of cell lines is a serious but often unrecognized problem. We describe the authentication of a panel of transitional cell carcinoma cell lines using the short tandem repeat profiling technique to detect cross-contamination. MATERIALS AND METHODS Genomic DNA was isolated from UM-UC-1, UM-UC-2, UM-UC-3 (ATCC), UM-UC-6, UM-UC-9, UM-UC-10, UM-UC-11, UM-UC-13, UM-UC-14,...
In this research seismicity parameters, repeat times and occurrence probability of large earthquakes are estimated for 35 seismic lineaments in Persian plateau and the surrounding area. 628 earthquakes of historical time and present century with MW>5.5 were used for further data analysis. A probabilistic model is used for forecasting future large earthquake occurrences in each chosen lineament....
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