The N-2 atom of guanine (G) is susceptible to modification by various carcinogens. Oligonucleotides with increasing bulk at this position were analyzed for fidelity and catalytic efficiency with the processive DNA polymerases human immunodeficiency virus, type 1, reverse transcriptase (RT), and bacteriophage T7 exonuclease(-) (T7(-)). RT and T7(-) effectively bypassed N(2)-methyl(Me)G and readi...

Effective gene therapy requires efficient delivery of DNA to transfected cells followed by high levels of gene expression. Our laboratory has developed two new delivery formulations LPDI containing the DNA condensing agent protamine sulfate and reconstitued chylomicrons containing a hydrophobic lipidDNA complex. LPDI can produce higher levels of gene expression than the corresponding liposome/D...

introduction pharmacologic treatment of radicular pain with oral medications is limited by adverse effects and concern for dependence. while topical formulations have been explored in pain research, there is no published literature evaluating the efficacy in radicular pain. we present the first three cases of radicular pain successfully treated with a topical formulation of diclofenac, ibuprofe...

paddy rice residue covers more than 60000 ha of the khuzestan plain. high soil moisture content (low evaporation and heavy soil texture), time limitations, and the likelihood of rainfall make conventional tillage methods unsuitable for wheat cultivation after the rice harvest. the present study investigated the best residue management method, and fuel and time savings, to produce acceptable yie...

Promoters for T7 RNA polymerase have a highly conserved sequence of 23 continuous base pairs located at position -17 to +6 relative to the initiation site for the RNA. The complex of T7 RNA polymerase with the phage 410 promoter has been visualizedindirectly by exploiting the ability of the polymerase to protect DNA sequences from cleavage by methidiumpropyl-EDTA-Fe(II). The DNA contacts made b...

Clinical history A 72 year old male patient presented with recent onset thoracic back pain. The pain was continuous and intense, exacerbated by movements of the spine, and radiated bilaterally to the costal region. The patient was known to suffer from non-insulin-dependent diabetes mellitus and hypertension. On admission, he appeared unwell and had a fever of 37.5'C. Clinical examination reveal...

Transcription of 17 DNA by purified Escherichia coli RNA polymerase without added factors produces long RNA molecules that begin near the left end of T7 DNA and terminate at the end of the early region. An endonuclease has been isolated from uninfected E. coli that cleaves these long RNAs at five specific sites to generate RNA molecules essentially the same as the early T7 RNAs observed in vivo...