The protein pattern of healthy human eccrine sweat was investigated and 10 major proteins were detected from which apolipoprotein D, lipophilin B, and cathepsin D (CatD) were identified for the first time in human eccrine sweat. We focused our studies on the function of the aspartate protease CatD in sweat. In vitro digestion experiments using a specific fluorescent CatD substrate showed that C...

After pilocarpine iontophoresis the change of sweat concentration during collection was studied by vapour pressure osmometry in 24 patients with cystic fibrosis and 24 healthy controls. There was a continuous but proportionate fall in sweat concentrations during the collection period. Mean (SD) initial sweat concentration in the control group was 154.4 (32.6) mmol/kg falling, after 50 microlite...

The concentrations of zinc, copper, iron, nickel, cadmium, lead, manganese, sodium and chloride in the sweat of six males and three females were determined after collections utilizing a total body washdown technique. From our results, sweat appears to be an important excretory pathway for zinc and copper. The mean concentrations of nickel and cadmium in sweat were higher than those reported for...

AIMS To determine whether the current practice of sweat testing in Swiss hospitals is consistent with the current international guidelines. METHODS A questionnaire was mailed to all children's hospitals (n = 8), regional paediatric sections of general hospitals (n = 28), and all adult pulmonology centres (n = 8) in Switzerland which care for patients with cystic fibrosis (CF). The results wer...

Effects of local skin temperature on sweat gland activity were analyzed quantitatively by measuring changes in the rates of thermal sweating and of drug-induced sweating by local heating. The data indicates that a rise in local temperature causes an accelerated increase in the rate of sweat production, the Q10 being around 2.5 regardless of the basal sweat rate with some individual variations. ...

BACKGROUND Collagen cross-link molecules such as pyridinoline (PYD), deoxypyridinoline (DPD), and N-terminal cross-linked peptides (NTX) have been measured in urine as indices of bone resorption. However, very little is known regarding the excretion of pyridinolines into other biological fluids. We report a collection device, normalizing analyte, and high-sensitivity immunoassay for quantitativ...

Six Angus steers (319 +/- 8.5 kg) were assigned to one of two groups (hot or cold exposure) of three steers each, and placed into two environmental chambers initially maintained at 16.5-18.8 degrees C air temperature (Ta). Cold chamber Ta was lowered to 8.4 degrees C, while Ta within the hot chamber was increased to 32.7 degrees C over a 24-h time period. Measurements included respiration rate,...

Introduction Anhidrosis is a decreased ability to sweat in response to increased body temperature. It most often occurs in horses living in hot, humid climates like Florida and other Gulf coast states. It is an important problem for performance horses because thermoregulation is mainly accomplished by sweating in the horse. Between 65-70% of heat is lost via the evaporation of sweat and the hum...

Most pediatricians eventually encounter a patient with a clinical presentation that warrants the consideration of a sweat test to rule out of confirm the diagnosis of cystic fibrosis. This article discusses, in a series of questions and answers, the currently available sweat testing methods and describes the various methods' reliability, limitations, and frequency of use. In addition, sweat tes...