In polarized epithelial MDCK cells, all known endogenous endocytic receptors are found on the basolateral domain. The influenza virus hemagglutinin (HA) which is normally sorted to the apical plasma membrane, can be converted to a basolateral protein by specific mutations in its short cytoplasmic domain that also create internalization signals. For some of these mutations, sorting to the basola...

in this study, mixed metal oxides al2o3/mgo/tio2 coatings with al/mg/ti ratio of 5:1:3 and 2.5:3:4 were coated on aluminum by sol-gel method. the surface morphology, phase analysis, thermal behavior and the corrosion behavior of the al2o3/mgo/tio2 coatings were characterized by (sem), (xrd), (tg-dta) and potentiodynamic polarization test in 3.5 wt.% nacl solution. the results demonstrated that ...

The spatial arrangement of COPII coat protein subunits was analyzed by crosslinking to an artificial membrane surface and by electron microscopy of coat proteins and coated vesicle surfaces. The efficiency of COPII subunit crosslinking to phospholipids declined in order of protein recruitment to the coat: Sar1p > Sec23/24p >> Sec13/31p. Deep-etch rotary shadowing and electron microscopy were us...

A halotolerant thermostable lipase was purified and characterized from the marine bacterium Oceanobacillus sp. PUMB02. This lipase displayed a high degree of stability over a wide range of conditions including pH, salinity, and temperature. It was optimally active at 30 °C and pH 8.0 respectively and was stable at higher temperatures (50-70 °C) and alkaline pH. The molecular mass of the lipase ...

This study tested the hypothesis that dietary cholesterol uptake by intestinal cells is dependent on the structure and composition of the lipid carriers in the extracellular milieu. In in vivo experiments with female C57BL/6 mice, cholesterol absorption from phospholipid/triacylglycerol emulsions was significantly reduced by administration of tetrahydrolipstatin, an inhibitor of pancreatic lipa...

Alpha-beta hydrolase domain-containing 5 (ABHD5), the defective gene in human Chanarin-Dorfman syndrome, is a highly conserved regulator of adipose triglyceride lipase (ATGL)-mediated lipolysis that plays important roles in metabolism, tumor progression, viral replication, and skin barrier formation. The structural determinants of ABHD5 lipolysis activation, however, are unknown. We performed c...

protective coatings that resist oxide scale growth and decrease chromium evaporation are necessary to makestainless steel interconnect materials for long-term durable operation of solid oxide fuel cells (sofcs). inthis study a layer of manganese was coated on the surface of aisi 441 ferritic stainless steel which is used insolid oxide fuel cells for interconnect applications. the oxidation beha...

A fiber coating from polyaniline (PANI) was electrochemically prepared and employed for Solid phase micreoextraction (SPME). The PANI film was directly electrodeposited on the platinum wire surface using cyclic voltametry (CV) technique. The same method was applied for the preparation of SPME fiber coated by polyaniline multiwalled carbon nanotubes (PANI/MWCNTs) composite. The concentration of ...

Marine fungus BTMFW032, isolated from seawater and identified as Aspergillus awamori, was observed to produce an extracellular lipase, which could reduce 92% fat and oil content in the effluent laden with oil. In this study, medium for lipase production under submerged fermentation was optimized statistically employing response surface method toward maximal enzyme production. Medium with soyabe...

A critical element of any stem cell differentiation protocol is the ability to compare its effects relative to an undifferentiated population of the same cells. In an attempt to standardize pre-differentiation conditions of adult derived mesenchymal stem cells prior to neural induction experiments, we asked what is the simplest chemical surface that supports the growth and maintenance of these ...