Analysis of proteins recovered in the S100 precipitate fraction of Streptomyces griseus after ultracentrifugation led to the identification of a 52-kDa protein which is produced during the late growth phase. The gene (eshA) which codes for this protein was cloned from S. griseus, and then its homologue was cloned from Streptomyces coelicolor A3(2). The protein was deduced to be 471 amino acids ...

AdpA in the A-factor regulatory cascade in Streptomyces griseus activates a number of genes required for secondary metabolism and morphological differentiation, forming an AdpA regulon. The Streptomyces subtilisin inhibitor (SSI) gene, sgiA, in S. griseus was transcribed in response to AdpA, showing that sgiA is a member of the AdpA regulon. AdpA bound a single site upstream of the sgiA promote...

Different organic solvents and detergents showed profound effects on the production of streptomycin (SM), amylases and proteases by Streptomyces griseus strains. The maximum SM production (1128 and 780 mg/L) was obtained using Streptomyces NRRL-strain when ethanol (1.0% v/v) and Tween-80 (0.1%v/v) were added to the medium. Production of amylases was also enhanced using the strain with the addit...

The coexistence of photosynthetic bacteria, lactic acid bacteria and Streptomyces, as required for the production of solutions of Effective Microorganisms (EM) is difficult, due to the different environments required by these species. However, the production of EM required that they coexist together, Hence studies were conducted to evaluate possible methods of developing an environment conduciv...

The spectinomycin (sp) resistance determinant from Streptomyces flavopersicus was cloned into Streptomyces lividans using the plasmid vector pIJ699. A plasmid, pDGL15, with a 3.65 kb insert from S. flavopersicus conferring resistance to Sp was isolated. DNA sequence analysis of the 3651 1 bp DNA insert revealed four open reading frames (ORFs). The amino acid sequence deduced from one ORF (SpcN)...

In our previous experiments (Arch. Microbiol. 40:261, 1951), no correlation was found between any of the morphological or physiological characteristics of various Streptomyces griseus strains and their streptomycin-producing capacity. There was but one common feature of those strains which did not produce streptomycin: they all had a short life cycle, which was also characterized by the early a...

In vitro tests of interactions between Streptomyces griseus strains and some soil borne plant pathogens like Fusarium oxysporum; Alternaria alternate; Rhizoctonia solani and Fusarium solani of tomato were studied on PDA medium. Strains tested produced a metabolite that inhibited growth of plant pathogenic fungi on PDA medium (Dual Culture Test). When grown in liquid cultures containing colloida...