Fibrates are hypolipidemic drugs that affect the expression of genes involved in lipid metabolism by activating peroxisome proliferator–activated receptors (PPARs). Fibrate treatment causes adverse changes in biliary lipid composition and decreases bile acid excretion, leading to an increased incidence of cholesterol gallstones. In this study, we investigated the effect of fibrates on bile acid...

It was known that the uptake of tryptophan is reduced in the yeast erg6 mutant, which is defective in a late step of ergosterol biosynthesis. Here, we show that this is because the high affinity tryptophan permease Tat2p is not targeted to the plasma membrane. In wild-type cells, the plasma membrane localization of Tat2p is regulated by the external tryptophan concentration. Tat2p is transporte...

BACKGROUND In contrast to the intense attention devoted to research on intracellular sterol trafficking in animal cells, knowledge about sterol transport in plant cells remains limited, and virtually nothing is known about plant endocytic sterol trafficking. Similar to animals, biosynthetic sterol transport occurs from the endoplasmic reticulum (ER) via the Golgi apparatus to the plasma membran...

The high sterol concentration in eukaryotic cell membranes is thought to influence membrane properties such as permeability, fluidity and microdomain formation. Drosophila cannot synthesize sterols, but do require them for development. Does this simply reflect a requirement for sterols in steroid hormone biosynthesis, or is bulk membrane sterol also essential in Drosophila? If the latter is tru...

Sterols are constituents of the cellular membranes that are essential for their normal structure and function. In mammalian cells, cholesterol is the main sterol found in the various membranes. However, other sterols predominate in eukaryotic microorganisms such as fungi and protozoa. It is now well established that an important metabolic pathway in fungi and in members of the Trypanosomatidae ...

Stearoyl CoA desaturase (SCD) is responsible for converting SFA into MUFA and plays an important role in regulating the fatty acid composition of tissues. Although the number of SCD isoforms differs among species, SCD-1 is the predominant isoform expressed in the major lipogenic tissues of all species studied. The SCD-1 gene promoter region has been cloned for several species, including the hum...

Mutants of Saccharomyces cerevisiae have been selected for resistance to the polyene antibiotics, etruscomycin, filipin, nystatin, pimaricin and rimocidin. All mutants are resistant to nystatin and there are several patterns of cross-resistance. The mutants were allocated to four unlinked genes pol I , 2, 3 and 5. A fifth gene, pol 4, was recovered as a double mutant with a strain carrying the ...

Due to its high price and consumer demand, virgin olive oil is an essential product vulnerable deception for unfair economic gain. In our research, sunflower, palm olein cottonseed were used as adulterants in different amounts the preparation of adulterated samples. Sterol composition, difference theoretical actual equivalent carbon number 42 triglycerides (ΔECN42) value FTIR spectra classify D...

In this study, Asphodelus tenuifolius (ATO) seeds oils were extracted by three different extraction methods, namely Soxhlet (SE), microwave-assisted (MAE) and ultrasonic-assisted (UAE). The physicochemical properties, fatty acids, sterols, tocols, total phenol content antioxidant activity of ATO seed examined compared. best yield was from SE (21.97 ± 0.01%), followed MAE (19.28 0.13%) while tha...

Sterols are major lipids in eukaryotes and differ in their specific structure between species. Both cholesterol and ergosterol can form liquid ordered domains in artificial membranes. We reasoned that substituting the main sterol ergosterol by cholesterol in yeast should permit domain formation and discriminate between physical and sterol structure-dependent functions. Using a cholesterol-produ...