نتایج جستجو برای: step pcr using specific primers
تعداد نتایج: 4365160 فیلتر نتایج به سال:
Abstract Background and Aims: The human papilloma virus was introduced as the major etiological agent in outbreak of cervical cancer in 1970. Since it is very difficult to recognize these viruses and their types using the serological tests and cell culture, molecular methods such as PCR are of great importance. Therefore, in this study, our goal was to use a multiple specific PCR assay on ...
Five fluorogenic probe hydrolysis (TaqMan) reverse transcriptase PCR (RT-PCR) assays were developed for serotypes 1 to 4 and group-specific detection of dengue virus. Serotype- and group-specific oligonucleotide primers and fluorogenic probes were designed against conserved regions of the dengue virus genome. The RT-PCR assay is a rapid single-tube method consisting of a 30-min RT step linked t...
Aim: The study was conducted to characterize and serotype the novel isolate of bluetongue virus (BTV) isolated from India. Materials and Methods: The BTV isolate was propagated in BHK-21 cell line. Nucleic acid (dsRNA) was extracted using Trizol method and cDNA was prepared using a process called reverse transcription. The cDNA was subjected to group specific PCR using ns1 gene specific primer ...
Abstract Pineapple is one of the essential export commodities in Indonesia. However, its production constrained by pathogen infections, including viruses. Recently, a field surveyed at pineapple area Blitar regency found virus-like symptoms such as leaf reddish intermingle with chlorosis and necrotic tips incidence up to 50% on clone 7350. until present, genetic identity causal viruses has been...
background: cd52 is a small glycoprotein with a gpi anchor at its c-terminus. cd52 is expressed by normal and malignant t and b lymphocytes and monocytes. there are detectable amounts of soluble cd52 in plasma of patients with cll and could be used as a tumor marker. although the biological function of cd52 is unknown but it seems that cd52 may be involved in migration and activation of t-cells...
Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using newly designed species-specific primers targeting mitochondrial 12S rRNA gene fragments. Methods: Two sets of specific primers were designed ...
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