The development and validation of a novel, simple, quick HPLC technique for measuring bosentan in pharmaceutical formulations was performed. parameters were tuned to be 1 mL/min flow rate, variable column temperature, mobile phase combination methanol-acetonitrile-water (20:50:30 v/v/v) carry out this study. All measurements carried with UV detector at wavelength 272 nm. Specificity, the limit ...