Vitellogenin (vtg) was isolated from greenback flounder (Rhombosolea tapirina), rainbow trout, (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar) plasma. The molecular weight of each native molecule, as determined by gel filtration, was 540, 383 and 557 kD respectively. With SDS-PAGE under reducing conditions, rainbow trout vtg dissociated into 1 major subunit (~154 kD), and Atlantic salmo...

A previous observation, using a few representative laboratory strains, that rabbit antisera raised against isolated surface fibrils might be useful in identifying Actinomyces viscosus and Actinomyces naeslundii isolates to their correct numerical taxonomy clusters was reexamined with a large culture collection, including clinical isolates from three different laboratories. Strains were first id...

Antigenic proteins of brain synaptic plasma membranes (SPM) and postsynaptic densities (PSD) were characterized using antisera raised against SPM. Immunostaining of brain sections showed that the antigens were restricted to synapses, and electron microscopy revealed staining at both presynaptic terminals and PSDs. In primary brain cell cultures the antisera were also neuron-specific but the ant...

This investigation determined the serum agglutination activity and serum bactericidal response after rabbit immunization with Pseudomonas paucimobilis. Agglutination activity of antisera showed a twofold increase in titer from before immunization to 4 weeks post-immunization and peaked at 8 weeks post-immunization with a titer of 1:512. 2-Mercaptoethanol reduction of immunoglobulin M decreased ...

ISBN 978 92 4 156348 2 The aim of this Consultative Meeting was to discuss strategies for improving the quality and quantity of therapeutic antisera, essential drugs for the effective treatment of suspected rabid dog bites and envenoming by snake bites and scorpion stings. Inadequacies in the effi cacy, safety and production of these antisera have created a major global public health crisis, es...

Somatic antigens of Bradyrhizobium japonicum, Rhizobium sp. (Cicer arietinum) and Rhizobium sp. (Leucaena leucocephala) were prepared as standard, single-species type from cultured cells. Equal numbers of the cells of these rhizobia were then combined to obtain a mixed-rhizobial-species antigen preparation. Rabbits were immunized either with the standard, single-species type or with the mixed-r...

this survey of salmonella and specifically salmonella enteritidis contamination in broiler flocks carried out in amol as one of the most important poultry breeding centers, used as manure, yolk sac, papers and visceral organs from dead birds. standard cultural methods were used for salmonella isolation. slide agglutination or tube agglutination tests were erformed using salmonella somatic o pol...

Antisera against whole cells of each Peptostreptococcus species (P. anaerobius, P. micros, P. parvulus, and P. productus) were produced in rabbits. When these antisera were reacted against sonically disrupted cells and culture supernatant fluids in Ouchterlony tests, lines of identity were obtained among the antigens from all the species and uninoculated culture medium. When the antisera were s...

Antisera directed toward the carboxyl-terminal region of human parathyrin (parathyroid hormone), for use in daignostically applicable radioimmunoassays of the hormone in serum, are scarce, largely because of the lack of suitable immunogens of human origin. We produced four antisera in goats and guinea pigs by immunization with recently discovered carboxyl-terminal fragments of human parathyrin ...

Specificity-associated determinants could be demonstrated on major histocompatibility complex (MHC)-restricted virus-immune cytotoxic T lymphocytes (CTL) using antisera raised in syngeneic mice. This result indicates that the number of clonotypes sufficiently small to allow the detection of such idiotypic determinants. The functional properties of three anti-idiotypic antisera were tested in bl...