The purpose of this study was to develop a biosensor based on surface plasmon resonance (SPR) for the rapid identification of C. jejuni in broiler samples. We examined the specificity and sensitivity of commercial antibodies against C. jejuni with six Campylobacter strains and six non-Campylobacter bacterial strains. Antigen-antibody interactions were studied using enzyme-linked immunosorbent a...

When designing liveness-enforcing Petri net supervisors, unlike other techniques, Li et al. added control places and arcs to a plant net model for its elementary siphons only, greatly reducing the structural complexity of the controlled system. Their method, however, suffers from the expensive computation of siphons. We propose a new T-characteristic vector z to compute strict minimal siphons (...

1University of Kassala, Kassala, Sudan. 2University of Khartoum, Khartoum, Sudan. New Halfa Hospital, New Halfa, Sudan (Correspondence to I. Adam: [email protected]). Received: 12/10/03; accepted: 17/05/04 ABSTRACT A prospective study investigated the epidemiology of malaria in an agricultural area in eastern Sudan from November 1999 to June 2000 (1 irrigation and 1 dry season). In monthly ...

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Troponin T (TnT) is a useful biomarker for studying drug-induced toxicity effects on cardiac cells. We describe how a surface plasmon resonance (SPR) biosensor was applied to monitor the release of TnT from active HL-1 cardiomyocytes in vitro when exposed to cardiotoxic substances. Two monoclonal human TnT antibodies were compared in the SPR immunosensor to analyse the TnT release. The detectio...

High-resolution differential surface plasmon resonance (SPR) with anodic stripping voltammetry (ASV) capability has been demonstrated for detecting heavy metal ions in water. Metal ions are electroplated onto the gold SPR sensing surface and are quantitatively detected by stripping voltammetry. Both the SPR angular shift and electrochemical current signal are recorded to identify the type and a...

In order to overcome the sensitivity limitation of SPR, nanoparticle-coupled SPR biosensors have explored with tremendous interests since nanoparticles may significantly enhance the sensitivity by 1-2 orders of magnitude. M. J. Natan et al. reported that a conjugate between an antibody and Au colloidal particle produced a greater than 20-fold increase in plasmon angle shift over than observed i...

The aim of this research is to characterize the optical properties of heavy metal ions (Hg, Cu, Pb and Zn) using surface plasmon resonance (SPR) technique. Glass cover slips, used as substrates were coated with a 50 nm gold film using sputter coater. The measurement was carried out at room temperature using Kretchmann SPR technique. When the air medium outside the gold film is changed to heavy ...

Surface plasmon resonance (SPR)-based biosensors were investigated with a view to providing a portable, inexpensive alternative to existing technologies for "real-time" biomolecular interaction analysis of whole cell-ligand interactions. A fiber optic SPR-based (FOSPR) biosensor, employing wavelength-dependent SPR, was constructed to enable continuous real-time data acquisition. In addition, a ...

Local injections of the neurotoxin SP-saporin into the basolateral amygdala (BLA) are reported to specifically lesion substance P receptor immunoreactive (SPR-IR) interneurons, and to reduce anxiety related behavior. Hence, this technique might provide a means to study how defined interneuron populations regulate neuronal activity in the BLA. However, what interneuron subgroups in the BLA might...