background: alkyl hydroperoxide reductase (ahpc) of helicobacter pylori is considered as a diagnostic antigen. there­fore, this antigen can be used to detect h. pylori infection by stool immunoassays such as elisa. the aim of this study was to simplify the ahpc protein purification procedures. methods: for whole cell protein extraction, the bacterial cells were ruptured by octly-β-d glucopyrano...

methods allplex gi-virus assay was developed to detect pathogens causing viral gastroenteritis, one of the major diseases caused by rna viruses. this one-step multiplex real-time polymerase chain reaction (pcr) based on the mudt technique permits simultaneous amplification and detection of target nucleic acids of norovirus gi, norovirus gii, rotavirus a, adenovirus f, astrovirus, and sapovirus ...

context correct timing of diagnostic laparoscopy for unexplained infertility management remains as a debate for clinicians. a cost-effective strategy of diagnostic laparoscopy utilization for unexplained infertile patients is much needed. evidence acquisition detailed evaluation of the articles extracted from a “pubmed” and “cochrane database” search using “unexplained infertility and diagnosti...

The Medicare rules governing the supervision of diagnostic tests have changed over time, as have the skills and training of radiologist assistants, who may have the education and clinical skills to deliver surgical and invasive procedures often performed as part of a radiology practice. However, for the purposes of Medicare coverage, radiologist assistants are not yet recognized as independent ...

Objective- The purpose of this study was to investigate the feasibility of performing echocardiography in apparently healthy pigeons, and measurement of the quantitative cardiac indices using B-mode and Doppler techniques. Design- Experimental study Animals- Ten apparently healthy pigeons Procedures- Echocardi...

This work shows that single sequencing reactions analyzed on an automated DNA sequencer can be an efficient way of screening PCR products for known mutations. We have analyzed a mutation in exon 10 of the human aromatase gene and show that an unambiguous genotype could be elucidated in more than 90% of the analyzed samples. Compared to analysis by full sequencing, 4 times more samples can be an...

A rapid and simplified fluorescence in situ hybridization (FISH) technique for the detection of human chromosome-specific centromeric probes is described. Using chromosomes 1-, 4-, 11and Y-specific fluorescence-labeled probes, the modified, or quick-FISH technique, was compared to two conventional FISH methods. The modified FISH technique detected human chromosomes without sacrificing sensitivi...

Accurate diagnosis is the prerequisite for effective treatment of diseases. Various biostatistics indices have been used to evaluate the performance of diagnostic tests. The distribution of test results commonly shows a relatively fixed overlap between the diseased and non-diseased populations, and the two main properties of laboratory tests sensitivity (Se) and specificity (Sp) usually demonst...