A Burdizzo castrator was evaluated for the neutering of dogs. Histological and morphological changes of spermatic cells and peripheral serum testosterone after challenge with a GnRH-analogue (gonadorelin) were assessed. There was a control group (G1), a surgically castrated group (G2) and a Burdizzo group (G3) divided in two, G3a receiving two crunches in each spermatic cord and G3b receiving o...

The sperm cell is designed to transmit the male DNA to the oocyte. The integrity of this DNA is an obvious requisite for normal embryo development and a successful pregnancy. Nevertheless, DNA quality, mainly the presence of DNA fragmentation, is not habitually assessed as part of sperm analysis due to the technical complexity of such an assessment and misunderstanding about its significance. C...

OBJECTIVES To obtain knowledge on male reproductive toxicity of inorganic lead at current European exposure levels and to establish lowest adverse effect levels, if any. METHODS A cross sectional survey of the semen of 503 men employed by 10 companies was conducted in the United Kingdom, Italy, and Belgium. The mean blood lead concentration was 31.0 microg/dl (range 4.6-64.5) in 362 workers e...

background: in previous investigations, it has been clarified that electromagnetic fields (elf) can cause some changes in cellular behavior. the aim of this prospective study was to investigate the effect of magnetic field (mf) on human sperm parameters of motility, morphology, and viability. materials and methods: semen samples were collected from 12 fertile men, and were allowed to liquefy fo...

Cryopreservation of caudal epididymal spermatozoa is an effective technique to conserve genetic potentials of superior dogs when it is not possible to collect ejaculated spermatozoa. Although hen egg yolk is commonly supplemented into the semen extender, active substances within the egg yolk which protect sperm against cryoinjury remain to be discovered. Among its compositions, low-density lipo...

OBJECTIVE To evaluate sperm nuclear DNA fragmentation and chromatin structure after 18 hours'incubation at room temperature. METHODS Twenty-eight male partners who participating IVF treatment were prospectively included in this study. Ejaculated sperm count and motility were assessed. The sperm was then immediately processed by the conventional swim-up method. After utilization of some of the...

OBJECTIVE To evaluate the effect of temperature on sperm motility, sperm motion characteristics, and production of reactive oxygen species (ROS) in semen. METHODS We collected semen samples from 12 infertile patients and 12 healthy donors and divided each sample into four aliquots just after liquefaction. One aliquot was analyzed immediately after liquefaction and the remaining three were ana...

YES of the dye or enzyme to the sites of damaged DNA) 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 TESTS of sperm DNA integrity have been used increasingly in the evaluation of the infertile man. These tests have been developed to further our understanding of spermatogenesis and sperm function, and to...

Objective: To determine if head and shoulder relaxation massage given immediately before collecting a semen sample will result in an increased total motile sperm count or improved semen parameters for intrauterine insemination (IUI). Design: Prospective randomized study Setting: Academic fertility center Patients: Forty men undergoing clomiphene IUI therapy with their partners Interventions: Ma...

Cooling method was proposed to maintain the sperm quality for several days. Nevertheless, during this procedure, sperm is encountered to “cold shock”, and its quality decreases time-dependently. This study was designed to improve the in vitro sperm preservation methods. Thirty normal semen samples were examined in Shiraz, Iran, 2017. Fifteen samples were incubated at 22-27 °C and 15 samples wer...