introduction: during spermiogendsis, sperm chromatin histones are replaced by protamine. after fertilization, the sprem nucleus is decondenced by glutathione (gsh). in this survey, we study the effect of various concentrations of gsh on human in vitro sperm decondensation. material and methods: after preparation and analysis of semen samples, sperm pellet was washed by ham's f-10 medium twice a...

OBJECTIVE To examine the relationship among sperm apoptosis, sperm chromatin status, and DNA ploidy in different sperm fractions. DESIGN Prospective study. SETTING Reproductive research center in a tertiary care hospital. INTERVENTION(S) Sperm prepared by density gradient were evaluated for sperm count, motility, apoptosis, and sperm chromatin assessment. MAIN OUTCOME MEASURE(S) Sperm c...

Spermatozoa generate reactive oxygen species (ROS) in physiological amounts which play a role in sperm functions during sperm capacitation acrosome reaction (AR) and oocyte fusion. In addition damaged sperm are likely to be the source of ROS. The most important ROS produced by human sperm are hydrogen peroxide superoxide anion and hydroxyl radicals. Besides human seminal plasma and sperm posses...

Our previous studies revealed an inhibitory effect of ICD-85 (Venom derived peptides) on breast cancer cell line MDA-MB231. ICD-85 was also confirmed by in-vivo studies to suppress the breast tumor in mice. However, the exact mechanism of ICD-85 was unknown. Hence, the present study was undertaken to assess the mechanism of ICD-85 effect as an anti-proliferative agent of cancer cells. The effec...

The monovalent ionophore monensin inhibits the secretion of both procollagen and fibronectin from human fibroblasts in culture. The distribution of these proteins in control and inhibited (5 x 10(-7) M monensin) cells has been studied by immunofluorescence microscopy. In control cells, both antigens are present throughout the cytoplasm and in specific deposits in a region adjacent to the nucleu...