OBJECTIVE Analyze whether testicular histologic patterns from a group of azoospermic men with varicocele is predictive of treatment outcome after subinguinal microsurgical varicocele repair. MATERIALS AND METHODS Seventeen azoospermic men underwent bilateral open single testis biopsy and microsurgical subinguinal repair of clinical varicoceles. RESULTS Histopathology of testicular biopsies ...

Centrosome integrity is critically important for successful fertilization and embryo development. In humans, the sperm contributes the dominant centrosomal material containing centrioles and centrosomal components onto which oocyte centrosomal proteins assemble after sperm incorporation to form the sperm aster that is essential for uniting sperm and oocyte pronuclei. Increasingly, dysfunctional...

BACKGROUND Aggressive immobilization of sperm prior to ICSI significantly improves fertilization rates, but the mechanism of this effect is not yet clear. This study was performed in order to assess the characteristics of mechanically immobilized human sperm by transmission electron microscope (TEM). METHODS Sperm obtained from ejaculated semen samples from three different donors were immobil...

We have examined the behavior of demembranated sperm heads when injected into the germinal vesicle (GV) of amphibian oocytes. Xenopus sperm heads injected into Xenopus GVs swelled immediately and within hours began to stain with an antibody against RNA polymerase II (Pol II). Over time each sperm head became a loose mass of chromosome-like threads, which by 24-48 h resolved into individually re...

AIM To investigate effects of quercetin on weight and histology of testis and accessory sex organs and on sperm quality in adult male rats. METHODS Male Sprague-Dawley rats were injected s.c. with quercetin at the dose of 0, 30, 90, or 270 mg/kg body weight/day (hereafter abbreviated Q0, Q30, Q90 and Q270, respectively), and each dose was administered for treatment durations of 3, 7 and 14 da...

Background: Rosa damascene constitutes showed different properties like antimicrobial, anti asthma and bronchitis, anti depression, and skin protection. Objective: Survey of effect of rose water extract on male mice reproductive parameter. Methods: In first part of experiment, adult male mice (Balb/c) 25 - 30 gram were divided into three groups; control, 10 and 20 ml/kg/day rose water. Intera...

Ten mature rams were paired on the basis of pre-treatment semen production and assigned randomly to one of two treatments. Treatments were bilateral intraepididymal injections of 3 ml of 50% (w/v) CaCI2 in .9% NaC1 (w/v) or 3 ml .9% NaCI. Semen was collected twice weekly for 5 weeks prior to treatment and for 7 weeks following treatment. Mounting times, ejaculate volume and sperm concentration ...

BACKGROUND In the majority of vertebrates, gametogenesis and gamete-release depend on the pulsatile secretion of luteinizing hormone-releasing hormone (LHRH) from the hypothalamus. Studies attempting to artificially stimulate ovulation and spermiation may benefit from mimicking the naturally episodic secretion of LHRH by administering priming injections of a synthetic analogue (LHRHa). This stu...

OBJECTIVE To examine the safety and effectiveness of assisted reproduction using sperm washing for HIV-1-serodiscordant couples wishing to procreate where the male partner is infected. DESIGN AND METHODS A retrospective multicentre study at eight centres adhering on the European network CREAThE and involving 1036 serodiscordant couples wishing to procreate. Sperm washing was used to obtain mo...