The in vitro synthesis and secretion of newly formed proteins were investigated in the principal cells of small pieces of the head and body of mouse epididymis inserted into a modified Rose chamber. Our ultrastructural observations revealed that the cells resumed their normal ultrastructural appearance only after 3 h. Therefore, after preliminary 3-hour culture, the pieces were incubated with t...

We present a new approach for biomass assessment in cell culture using a disposable microcentrifuge tube. The specially designed tube is fitted with an upper chamber for sample loading and a lower 5 microL capillary for cell collection during centrifugation. The resulting packed cell volume (PCV) can be quantitatively expressed as the percentage of the total volume of the sample. The present st...

children and in children with renal disease. More accurate measurement was possible, particularly if a 4-hour specimen was used rather than an overnight 12-hour specimen. However, there are so many variable errors in any method of estimating cell excretion rates, that the method could well be discarded in paediatric practice. Cell concentration in a random urine specimen is easily estimated, a ...

The effect of psilostachyin, a natural sesquiterpene lactone, on the growth and viability of cultured epimastigotes of Trypanosoma cruzi (Tulahuen) is reported. The antiproliferative effect was evaluated by counting the parasites in a Neubauer chamber and measuring their viability by using the dye exclusion technique. The effect on parasite growth was irreversible at concentrations higher than ...

Fluorescence microscopy of cells loaded with fluorescent, Ca2+-sensitive dyes is used for measurement of spatial and temporal aspects of Ca2+ signaling in live cells. Here we describe the method used in our laboratories for loading suspensions of human sperm with Ca2+-reporting dyes and measuring the fluorescence signal during physiological stimulation. Motile cells are isolated by direct swim-...

A fully automated and fast pneumatic transport system for short-time activation analysis was recently developed. It is suitable for small nuclear research reactors or laboratories that are using neutron generators and other neutron sources. It is equipped with a programmable logic controller, software package, and 12 devices to facilitate optimal analytical procedures. 550 ms were only necessar...

A 30-year-old female presented with a one-month history of blurred vision in her left eye. Examination revealed a live motile worm in the anterior chamber of the left eye. She also had retinal pigment epithelial disturbance with focal intraretinal haemorrhage. The 19.9 mm worm was surgically removed and identified as Angiostrongylus cantonesis. She was treated with oral mebendazole. Her vision ...

A rapid method for directly determining the exhalation rate of thoron from material surfaces was studied. Thoron accumulated with the accumulation chamber is detected with the scintillation cell. The alpha counting efficiency of the scintillation cell was estimated by the Monte Carlo calculation. Measurements were carried out in a sandy loam and different building materials. The results show th...

The 4th Concept detector presently being designed for the International Linear Collider introduces several innovations in order to achieve the necessary experimental goal of a detecter that is 2-to-10 times better than the already excellent SLC and LEP detectors. We introduce a dual-readout calorimeter system, a cluster counting drift chamber, and a second solenoid to return the magnetic flux w...

A technique has been developed to determine the absolute single particle counting efficiency of a channel electron multiplier (CEM) for positively charged ions at keV energies. The calibration technique is applicable to positive ion detectors in general. Ion beam currents of C?+ were created by charge transfer of C? on H, in the thin target regime. By adjusting the Hz pressure in the beam scatt...