The effects of acute restraint stress on sperm motility and reproductive endocrinology were investigated in adult rats. Sperm motility was determined by computer-assisted sperm analysis. Acute restraint stress reduced sperm motility starting after 30 min, and the sperm motility parameters, percentage of motile spermatozoa (%), straight-line velocity, curvilinear velocity, deviation of the sperm...

BACKGROUND A high-fat diet (HFD) promotes the oxidative stress formation, which in turn has hazardous effects on reproductive system and fertility. The present study examines the potential positive effects of a restricted high-fat diet (RHFD) and antioxidants consumption on sperm parameters and testis tissue in rats. METHODS Male rats (n = 48) were divided into four groups (12 in each group):...

Infertility is considered as one of the major problems associated with spinal cord injury (SCI). However, the exact underlying mechanism is still unknown. Therefore, the main objective of this experimental study was to evaluate the effect of chronic SCI on sperm parameters as well as chromatin integrity and DNA of spermatozoa aspirated from cauda epididymis of rats. Forty-five adult Wistar rats...

Sperm mediated gene transfer (SMGT) could provide the opportunity to carry out transgenesis on a mass scale using spermatozoa as vectors for exogenous DNA. However, the efficiency of sperm-mediated DNA transfer is still questionable, and the mode of transmission to the egg has not yet been well understood. Our aim was to investigate the capacity of bovine spermatozoa to carry exogenous DNA and ...

BACKGROUND Semen quality, a predictor of male fertility, has been suggested declining worldwide. Among other life style factors, male coffee/caffeine consumption was hypothesized to influence semen parameters, but also sperm DNA integrity. To summarize available evidence, we performed a systematic review of observational studies on the relation between coffee/caffeine intake and parameters of m...

introduction: to evaluate the quick cryopreservation technique on parameters of motility, morphology, and vitality of spermatozoa from normozoospermic ejaculates. materials and methods: in this prospective study, a total of 37 normal ejaculates were evaluated according to who criteria, then each sample was divided into two aliquets and sample was cryopreserved with quick freezing method. sperm ...

This study was aimed at evaluating the in vitro effects of particulate matter (ranging from 2.5-10 m in an aerodynamic diameter, PM10) from an urban area on the motility of human spermatozoa. Samples of swim up selected human spermatozoa from three healthy men with normal semen parameters, according to WHO guidelines, were incubated for 4 hours with 10, 50 and 75 μg/ml of PM10. Kinetic paramete...

OBJECTIVE To compare the semen quality and age-specific changes in men between the 1980s and 2000s. DESIGN Prospective study. SETTING Andrology laboratory, University of Calcutta, India. PATIENT(S) A semen sample was obtained from 3729 men presenting for infertility problems in two distinct decades, that is, between 1981-85 and 2000-2006. INTERVENTION(S) Subjects with sperm count >20 x ...

38 fresh ejaculates were examined, of which 23 were cryopreserved in three diluents differing in concentration of glycerol and examined after thawing. For each ejaculate we established the concentration, motility, speed of sperm, percentage of live sperm and the morphological image of the ejaculate using routine laboratory diagnosis and SQA parameters at the initial value and after a 120-minute...

Adriamycin (ADR), an anticancer drug which induces testicular toxicity, was administrated to Slc:SD male rats at doses of 0.5, 1.0, and 2.0 mg/kg intravenously once a week for 4 weeks. The males treated with ADR were mated with untreated females, and sperm analyses (motion, count, and morphology) were performed. Sperm motion was analyzed by Hamilton-Thorne Sperm analyzer (HTM-IVOS) to investiga...