Pathogenic bacteria secrete various virulence factors, including toxins, lipases and proteases that allow them to infect, breakdown and colonize host tissue. Among various modes of action that the pathogenic bacteria use to damage the host, pore formation (by pore forming toxins (PFTs)) and lipid hydrolysis (by phospholipases) modes are common in damaging the eukaryotic cell membrane. PFTs in t...

Here, we first provide a facile ultrasonic-assisted synthesis of SnO using SnCl2 and the organic solvent of ethanolamine (ETA). The moderate alkalinity of ETA and ultrasound play very important roles in the synthesis of SnO. After the hydrolysis of the intermediate of ETA-Sn(II), the as-synthesized SnO nanoclusters undergo assembly, amalgamation, and preferential growth to microplates in hydrot...

We demonstrate the use of tethered bilayer lipid membranes (tBLMs) as an experimental platform for functional and structural studies of membrane associated proteins by electrochemical techniques. The reconstitution of the cholesterol-dependent cytolysin (CDC) pyolysin (PLO) from Trueperella pyogenes into tBLMs was followed in real-time by electrochemical impedance spectroscopy (EIS). Changes of...

Introduction: Electrical impedance spectroscopy (EIS) is a non-invasive method for characterising human tissue. Because currents will pass either around or through cells depending on the frequency, EIS can be used to observe the structure and arrangement of cells. EIS has been used to identify precancerous changes in the oesophagus and cervix [1]. Many of the precancerous changes in tissue conc...

Non-invasive glucose monitoring is crucial for effective diabetes mellitus treatment while a sound correlation of a non-invasive parameter to glucose level variation is quite challenging. This paper presents characterization of glucose concentrations using Electrical Impedance Spectroscopy (EIS) in three different solutions: 1) 0.9% NaCl, 2) Saline (NaCl 1.3gm, KCl 0.75gm, Na3C6H5O7 1.45gm, D-g...

Polypyrrole (PPy) films of different thickness were characterized by electrochemical impedance spectroscopy (EIS) measurements in acetonitrile and aqueous solutions, containing 0.1 M NaClO4 or sodium dodecylsulfate as the dopant. The PPy films were electrochemically deposited on Pt, and their electrochemical properties studied by cyclic voltammetry. Impedance spectra were obtained at potentials...

In this article we describe the integration of impedance spectroscopy (EIS) and surface plasmon resonance (SPR) into one surface analytic device. A polydimethylsiloxane (PDMS) flow cell is created, matching the dimensions of a commercially available sensor chip used for SPR measurements. This flow cell allowed simultaneous measurements between an EIS and a SPR setup. After a successful integrat...

The validity of estimating the solid phase diffusion coefficient, D s , of a lithium intercalation electrode from impedance measurement by a modified electrochemical impedance spectroscopy ~EIS! method is studied. A macroscopic porous electrode model and concentrated electrolyte theory are used to simulate the synthetic impedance data. The modified EIS method is applied for estimating Ds . The ...

Non-linear Harmonic Analysis is used to investigate the distortions accompanying Electrochemical Impedance Spectroscopy (EIS) measurements. The emerge from non-linearity and non-stationarity due underlying redox reactions as well capacitance. Identifying each response its source crucial correlate obtained harmonics their corresponding electrochemical processes. In this study, we identify of Gal...

Here, we proposed to test the limits of bounded diffusion impedance model by using electrochemical spectroscopy (EIS) measurements in a microfluidic device architecture bearing set microelectrodes integrated microchannel. This EIS Nyquist plot behaviour was currently modelled with Randles equivalent circuit. In this work, one main advantages for PyEIS (a Python-based Electrochemical Impedance S...