Glycosphingolipids (GSL's) belong to the glycoconjugate class of biomacromolecules, which bear structural information for significant biological processes such as embryonic development, signal transduction, and immune receptor recognition(1-2). They contain complex sugar moieties in the form of isomers, and lipid moieties with variations including fatty acyl chain length, unsaturation, and hydr...

The purpose of the work is to substantiate method determining collagen in food products. paper substantiates ratio sample mass and distilled water during removal gluten, temperature duration tested developed for by assessing content samples small-mouthed sea smelt.
 Theoretical, physico-chemical, spectrometric mathematical research methods were used work.

This thesis consists of five parts. In the first part, an automated method for quantitative analysis of phospholipid compositions of cells and tissues by liquid chromatographymass spectrometry was developed. In the second part, this method was applied to investigate brain lipid compositions of patients with progressive epilepsy with mental retardation (EPMR), caused by mutations in the CLN8 gen...

Driving under the influence of prescribed or illegal drugs increases the risk of having road accidents, just like driving under the influence of alcohol. In forensic toxicology, an increasing number of blood samples must be analyzed for drugs. Immunoassays tailored for a limited number of drugs (of abuse) are usually applied as prescreening tests at the roadside and/or in the laboratory. Howeve...

Classically, experiments aimed at studying changes in protein expression have always followed a small set of proteins. This focused approach was necessary since tools to efficiently analyze large numbers of proteins were simply not available. Large-scale quantitative proteomics promises to produce reams of data that previously would have taken decades to measure with classical methods. Mass spe...

a convenient liquid chromatographic-single quadrupole mass spectrometric (lc-ms) method was developed and validated for dexchlorpheniramine maleate (inn name: chlorphenamine) determination in human plasma. the need for just a single liquid-liquid extraction with ethyl acetate and being highly sensitive were the advantages of this method. the linearity was also excellent over the range of 1 to 1...

An electrospray ionization-mass spectrometric procedure has been developed for determining glycohemoglobin. Whole-blood samples from 78 diabetic and 50 nondiabetic subjects (glycation range 3-15%, as determined by electrospray mass spectrometry) were diluted 500-fold in an acidic denaturing solvent and introduced directly into a mass spectrometer. The resulting mass spectra were then processed ...

Since there is an enormous volume of information on the separation of antibiotics in the literature, readers should be able to Rnd HPLC conditions for almost any antibiotic of interest. Readers are also encouraged to consult the ofRcial compendia for analysis of bulk or formulated drugs. For analysis of biological samples, the samples may be directly injected with a column switching technique i...

An analytical assay has been developed and validated for ultrafast and high-throughput mass spectrometric determination of pemetrexed concentrations in plasma using matrix assisted laser desorption/ionization-triple quadrupole-tandem mass spectrometry. Patient plasma samples spiked with the internal standard methotrexate were measured by multiple reaction monitoring. The detection limit was 0.4...