the genus artemisia containing more than 500 taxa at the specific or subspecific level. thirty-four species of genus artemisia have been reported in iran, which from the viewpoint distribution, density and coverage is the most important genera of plant. since the still has not been achieved a general system for the classification and phylogeny, in this research tried with evaluation of chloropl...

Prevotella oris is a nonpigmented, Gram-negative, anaerobic bacterium that has been associated with several serious oral and systemic infections. Prevotella oris has been identified in clinical specimens by bacterial culture and biochemical tests, which are generally unreliable. The aim of this study was to develop a PCR assay for the direct detection of P. oris in clinical specimens. PCR prime...

The ultra-fast quantitative real-time polymerase chain reaction (qPCR) assay was developed and validated to differentiate the morphologically similar ones, Oncorhynchus keta mykiss. Species-specific primers were designed for COI genes of mtDNA. species-specific O. mykiss selectively amplified by DNA, respectively. sensitivity 1 ng/μL. Quantitative testing showed that results met ‘Guidelines on ...

Bioluminescence is reported in members of 18 dinoflagellate genera. Species of dinoflagellates are known to have different bioluminescent signatures, making it difficult to assess the presence of particular species in the water column using optical tools, particularly when bioluminescent populations are in nonbloom conditions. A "universal" oligonucleotide primer set, along with species and gen...

in sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. in the present study we empl...

in sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. in the present study we empl...

We have developed a combinatorial polymerase chain reaction (PCR) procedure to identify four major species of the genus Brucella simultaneously. Four pairs of primers targeting the genes encoding a cell surface protein (BCSP31) and outer membrane proteins (omp2b, omp2a and omp31) were prepared. PCR using these primers gave rise to specific patterns of amplification for each Brucella spp. examin...

Different species of Trichoderma especially T. harzianum (Th2 biotype in Europe and Th4 in America) considered as causal agent of green mould disease on button mushroom (Agaricus bisporus) casing beds. In this study, 100 fungal isolates were collected and purified from different mushroom growing units in Tehran province and Urmia (NW Iran). Totally, 60 isolates of T. harzianum, 27 isolates of T...