Methods were studied for the cryopreservation of microfilariae of periodic Brugia malayi. RPMI-1640 tissue culture medium containing 6% dimethyl sulfoxide (DMSO) and 15% newborn calf serum was used as cryoprotectant. Samples were frozen slowly in the vapor phase of liquid nitrogen prior to emersion in liquid nitrogen (-196 degrees C). The freezing rate was -0.5 to -1.0 degrees C per minute, mic...

Feasibility will take into account whether it is possible to operate the freezer in tile plant location. A liquid nitrogen freezer (LNF), for instance, may be suitable in every respect for freezing the product and the high costs of using this method of freezing may be justified. However, if the location of the plant is such that there can be no guaranteed supply of liquid nitrogen, the freezer ...

The electrical conductivity of beryllium at radio frequency (800 MHz) and liquid nitrogen temperature were investigated and measured. This summary addresses a collection of beryllium properties in the literature, an analysis of the anomalous skin effect, the test model, the experimental setup and improvements, MAFIA simulations, the measurement results and data analyses. The final results show ...

abstract in order to evaluate the effect of different nitrogen application methods on yield and yield components of winter wheat, a field experiment with split plot arrangement in completely randomized blocks design with four replicates was conducted in 2006. basal dressing in three levels (1- with no basal dressing, 2- strip and 3- distributing nitrogen fertilizer) as main plot and top dressin...

Using liquid helium, liquid nitrogen, and water as test fluids, we attempt to generate homogeneous turbulence in a steadily rotating system. We create turbulence by pulling a grid in rotating channels with both square and round cross sections, and observe large-scale inertial waves in the flow. These inertial waves quickly sense the boundaries, and resonate at frequencies characteristic of the ...

Conservation of genetic resources is connected with numerous problems which solution is crucial for the success of the entire program. At present the only effective method of long-term conservation of genetic material is its deep freezing in liquid nitrogen at -196°C. Some positive results were also obtained with the use of liquid oxygen which allows the use of lower temperatures. Cryo-conserva...