plant tissue culture techniques are used as basic requirement of common plant transformation systems. in most cases of plant transformation, a reproducible regeneration protocol is the limiting step due to long time lasting, specialized facilities and well experienced persons. furthermore, tissue culture procedures induce somaclonal variation among regenerated transgenic plants. therefore, rece...

In the present study, Randomly Amplified Polymorphic DNA (RAPD) markers were used to analyze the genetic diversity in 20 varieties of Philodendron. The polymerase chain reaction was performed with 60 RAPD primers, out of which 21 primers showed clear amplification as well as more polymorphism. In total, 354 scorable RAPD loci with 348 polymorphic bands (98%) were observed. Percentages of polymo...

nowadays, tobacco is considered as one of the important world industrial plants. in the present study, for a creation of somaclonal variation, calli were produced on ms medium supplemented by 2 mg/l of naphthalene acetic acid (naa) and then regenerated on ms medium supplemented with 1 mg/l benzyl amino purine (bap) and 0.1 mg/l of naa. to determine correlations among traits and to fit multiple ...

Sipahutar pineapple is very popular in North Sumatra, because of the distinctive sweet taste and normal water content. Furthermore, it large size has a yellow fruit skin color with greenish tips. However, problem production limited amount good quality seeds. The utilization vitro culture techniques on pineapples allows emergence somaclonal variations, especially plantlets that have undergone su...