A large set of genome-wide markers and a high-throughput genotyping platform can facilitate the genetic dissection of complex traits and accelerate molecular breeding applications. Previously, we identified about 0.9 million SNP markers by sequencing transcriptomes of 27 diverse alfalfa genotypes. From this SNP set, we developed an Illumina Infinium array containing 9,277 SNPs. Using this array...

Sufficient codominant genetic markers are needed for various genetic investigations in alfalfa since the species is an outcrossing autotetraploid. With the newly developed next generation sequencing technology, a large amount of transcribed sequences of alfalfa have been generated and are available for identifying SSR markers by data mining. A total of 54,278 alfalfa non-redundant unigenes were...

The objectives of this study were to expand the database and determine the intestinal digestibility of rumen undegradable protein (dRUP) of common dairy feeds and to determine the effects of feeding 37.9% wet corn gluten feed on these estimates. Two ruminally and duodenally fistulated steers were assigned randomly to a crossover design with 4-wk periods. The mobile bag technique was used to det...

Building a 2,000-square-foot house leaves about a ton of gypsum wallboard waste from end cuts, window and door cutouts, and broken boards. In the United States, three million tons of gypsum wallboard waste is dumped in landfills each year. In addition to depleting space, microbial action can decompose gypsum to malodorous hydrogen sulfide gas, which at high enough exposures can cause irritation...

the current experiment was carried out to investigate the possible effects of replacing alfalfa by dried de-oiled savory (satureja khuzistanica; dds) at the levels of 0, 25, 50, 75 and 100 percent in finishing diets of mehraban lambs on kinetics of gas production in vitro. the experimental diets contained 20% alfalfa and 80% of concentrate on dry matter basis. replacing alfalfa by dds had no si...

Cytokinin is required for the initiation of leguminous nitrogen fixation nodules elicited by rhizobia and the delay of the leaf senescence induced by drought stress. A few free-living rhizobia have been found to produce cytokinin. However, the effects of engineered rhizobia capable of synthesizing cytokinin on host tolerance to abiotic stresses have not yet been described. In this study, two en...

The present work was planned to evaluate the phytoextraction of metal mine tailings, Sarcheshmeh copper mine, SE of Iran, by the endemic plant species Medicago sativa L. (Alfalfa). In this pot experiment, we investigated the effects of seven amendments on the growth of alfalfa and uptaking metals from the mine tailings and stream sediment of tailing dam surface. The mean metal concentrations in...

This paper reviews the current status, methodology, achievements, and prospects of alfalfa (Medicago sativa L.) breeding in China. There are 77 cultivars that have been registered in the country, these include 36 cultivars bred through breeding programs, 17 introduced from overseas, 5 domesticated from wild ecotypes, and 19 through regional collection/breeding programs. Cultivars have been sele...

Cold acclimation in alfalfa (Medicago sativa L.) plays a crucial role in cold tolerance to harsh winters. To examine the cold acclimation mechanisms in freezing-tolerant alfalfa (ZD) and freezing-sensitive alfalfa (W5), holoproteins, and low-abundance proteins (after the removal of RuBisCO) from leaves were extracted to analyze differences at the protein level. A total of 84 spots were selected...

Winter damage, especially in northern climates, is a major limitation of the utilization of perennial forages such as alfalfa. Therefore, improving freezing tolerance is imperative in alfalfa genetic breeding. However, freezing tolerance is a complex trait that is determined by many genes. To understand the complex regulation mechanisms of freezing tolerance in alfalfa, we performed small RNA s...