multiple sclerosis (ms) is a demyelinating disease of the central nervous system characterized by morphological hallmarks of inflammation, demyelination and axonal loss. to date, little attention has been paid to the contribution of mitochondrial respiratory chain enzyme activities to ms. in this study, kinetic analysis of mitochondrial respiratory chain complex i enzyme (measured as nadh ferri...

Muscle stem cells (MuSCs) hold great therapeutic potential for muscle genetic disorders, such as Duchenne muscular dystrophy (DMD). The CRISP/Cas9-based genome editing is a promising technology for correcting genetic alterations in mutant genes. In this study, we used fibrin-gel culture system to selectively expand MuSCs from crude skeletal muscle cells of mdx mice, a mouse model of DMD. By CRI...

A 6-year survey (1 October 2006 – 1 October 2012) on the prevalence of developmental skeletal abnormalities in dogs was performed based on patients’ records of the Small Animal Clinic to the Faculty of Veterinary Medicine – Stara Zagora, Bulgaria. From the total number of 6,097 dogs with surgical disorders, developmental skeletal disorders were diagnosed in 230 dogs (3.78%). The incidence of di...

Denosumab (AMG 162) is an investigational fully human monoclonal antibody with a high affinity and specificity for receptor activator of nuclear factor-kappaB ligand (RANKL), a cytokine member of the tumor necrosis factor family. RANKL, the principal mediator of osteoclastic bone resorption, plays a major role in the pathogenesis of postmenopausal osteoporosis and other skeletal disorders assoc...

Maintaining skeletal muscle function throughout the lifespan is a prerequisite for good health and independent living. For skeletal muscle to consistently function at optimal levels, the efficient activation of processes that regulate muscle development, growth, regeneration and metabolism is required. Numerous conditions including neuromuscular disorders, physical inactivity, chronic disease a...

Dielectrophoresis (DEP) is a non-invasive cell analysis method that uses differences in electrical properties between particles and surrounding medium to determine a unique set of cellular properties that can be used as a basis for cell separation. Cell-based therapies using skeletal stem cells are currently one of the most promising areas for treating a variety of skeletal and muscular disorde...

Skeletal muscle fibers differ considerably in their metabolic and physiological properties. Skeletal muscle displays a high degree of metabolic flexibility, which allows the myofibers to adapt to various physiological demands by shifting energy substrate utilization. Transcriptional events play a pivotal role in the metabolic adaptations of skeletal muscle. The expression of genes essential for...

conclusion a significant relationship exists between the type of facial skeletal classification and the shape of the sella turcica; as in class iii patients, sella turcica bridge was reported with a higher frequency. also, sella turcica had a significantly higher length in these patients than in those with class i and class ii facial skeletal types. results sella turcica had normal morphology i...

Cornelia de Lange syndrome (CdLS) is a rare genetic disorder that is characterized by specific facial, skeletal, and behavioral features associated with variable degrees of intellectual disabilities. Sleep disturbances have been reported in patients with CdLS including insomnia, sleep-disordered breathing, intrinsic sleep disorders, and circadian rhythm disorders. The prevalence of sleep-relate...

Coenzyme Q (CoQ) is a ubiquitous lipid serving essential cellular functions. It the only component of mitochondrial respiratory chain that can be exogenously absorbed. Here, we provide an overview current knowledge, controversies, and open questions about CoQ intracellular tissue distribution, in particular brain skeletal muscle. We discuss human neurological diseases mouse models associated wi...