Background: PhiC31 integrase system provides a new platform in various felid of research, mainly in gene therapy and creation of transgenic animals. This system enables integration of exogenous DNA into preferred locations in mammalian genomes, which results in robust, long-term expression of the integrated transgene. Objectives: Identification of a novel pseudo attP site. Materials and Methods...

The parafascicular nucleus (PFN) of thalamus, as a supraspinal structure, has an important role in processing of nociceptive information. In addition, μ-opioid receptor contributes to supraspinal modulation of nociception. In the present study, the effects of microinjection of naloxone (a non-specific opioid-receptor antagonist) and naloxonazine (a specific μ-opioid receptor antagonist) were in...

Despite the critical role of erythropoietin (EPO) as therapeutic agent in treatment of anemia, its consumption is limited due to several disadvantages including the product short half-life, immunogenicity and susceptibility to proteolytic degradation. To overcome these drawbacks efficient methods such as site-specific PEGylation have been developed among witch N-terminal PEGylation has found mo...

We present a database-centric approach to web site development in which both application and web content data are managed by a database. The development process is based on three main stages of information modelling, document content design and presentation specification. A Java framework based on the OMS object-oriented data management system has been developed to support the development life ...

We present a new, practical algorithm to resolve the experimental data in restriction site analysis, which is a common technique for mapping DNA. Specifically, we assert that multiple digestions with a single restriction enzyme can provide sufficient information to identify the positions of the restriction sites with high probability. The motivation for the new approach comes from combinatorial...

Building data-intensive Web sites and especially Web portals is a costly task, which requires considerable effort for data integration and maintenance and usually does not result in many reusable components. This is mainly because most of the design is hard-coded in static or dynamic Web pages. In this paper we integrate three different approaches to create a comprehensive solution to Web site ...

This paper presents the aerodynamic design and economical evaluation of a 200 kW site specific wind turbine for the province of Semnan in Iran. By designing site specific wind turbines, the cost of energy which is calculated out of the annual energy production and also the cost of manufacturing the rotor are minimized. The aerodynamic design is based on the well-known blade element momentum...

Evaluating agricultural land management practices requires knowledge of soil spatial variability and understanding their relationships. Spatial distributions for fourteen soil physical and chemical properties were examined in a wheat field in Sorkhankalateh district, in Golestan province, Iran. 101 soil samples at the distances of 5m, 10m and 20m as nested grid were collected at the depth of 0-...