Background & Aims: Many studies have demonstrated that Vitamin D has an important role in cell growth and proliferation and vitamin D receptor polymorphism has significant relationship with colorectal cancer (CRC). The aim of this study was to assess the incidence of VDR rs 2238136 polymorphism in Iranian population and to investigate the relationship between this single nucleotide polymorphism...

background chronic hepatitis b virus (hbv) infection is a multifactorial disease that can result in serious clinical complications. host genetic background especially the genes that encode immunologic factors like inf-γ and its receptor (ifn-γ r) are critical in the pathogenesis of infection. materials and methods genomic dna from peripheral blood samples of 200 chronically hbv infected patient...

Allele-specific polymerase chain reaction (AS-PCR) is a rapid and cost-effective single nucleotide polymorphism (SNP) genotyping method compared to multiplex or real-time PCR. The SNPs occurring in mitochondrial DNA (mtSNPs) the most abundant humans can be used human identification involving mass fatality incidents. Nevertheless, application of AS-PCR has yet widely applicable forensic investig...

Reproduction in domestic animal is a very complex process. The process starts from gamete formation, release, transport, fertilization, embryo and fetal development until delivery of the offspring by dam. holds most important role determining success livestock farming, profit or loss. genetic selection reproductive traits became an program to find out merit candidate dam bulls. At early time, f...

This research aimed to assess the genetic polymorphism of BMP15 gene concerning sex hormone levels and birth type in Awassi ewes. The genome DNA 138 ewes was isolated (52 produced a singleton 86 twins). exon-2 amplifiable by polymerase chain reaction (PCR), so two genotypes were identified based on 141 bp amplicons: TT TA. A sequencing revealed novel mutation, c.50980646T>A, TA genotype. single...

the melanocortin-1 receptor mc1r is encoded by the extension locus, playing a fundamental role in the determination of coat color in a number of mammalian species. however, so far there has been no report regarding the single nucleotide polymorphisms (snps) of the mc1r promoter region and the potential association of its snps with coat color in sheep (ovis aries). throughout the present study, ...

Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) is a simple, timeand cost-effective SNP genotyping method. In this study, we propose a MA (memetic algorithm)-based method to design a feasible CTPP primer set. Overall, 288 SNPs in the SLC6A4 gene were tested in silico by the proposed method. The results indicate that the proposed method provides feasible CTPP primers that ...

MOTIVATION Oligonucleotide microarrays allow genotyping of thousands of single-nucleotide polymorphisms (SNPs) in parallel. Recently, this technology has been applied to loss-of-heterozygosity (LOH) analysis of paired normal and tumor samples. However, methods and software for analyzing such data are not fully developed. RESULT Here, we report automated methods for pooling SNP array replicate...

Abstract The hazel dormouse Muscardinus avellanarius presents an exemplary non-model species that is both locally threatened and whose genetic status not fully understood owing to insufficient resolution of the currently available molecular tools. We performed normalized Genotyping-by-Sequencing (nGBS) on 48 samples collected across European distribution, aiming at discovering useful single nuc...

results the analysis of data for c/t snp showed that the cc genotype is more common in the control group than in the group of patients. in contrast, the frequency of tt as a mutant genotype is more frequent in patients than in uninfected people. in addition, results showed a statistically significant relationship between cc, ct, and tt genotypes in sensitive and resistant groups (p value: < 0.0...