نتایج جستجو برای: single chain variable fragment scfv

تعداد نتایج: 1405110  

2018
Lin Xin Hou-Ting Zhang Wei-Feng Yang Yi-Fan Li Chuan Liu

PEG-PLGA nanoparticles (NPs) modified with anti-CD133 and tumor-targeting single-chain antibody fragment (scFV-NPs) for systemic delivery of methioninase (METase) and pemetrexed for gastric carcinoma were successfully formulated. The structure characterization and biological functions of METase-pemetrexed-loaded scFV-PEG-PLGA NPs (scFV-METase/pemetrexed-NPs) in vitro were investigated. Function...

Journal: :Vaccine 2010
Tao Zhang Cheng-Yu Wang Wei Zhang Yu-Wei Gao Song-Tao Yang Tie-Cheng Wang Ren-Zhou Zhang Chuan Qin Xian-Zhu Xia

The hemagglutinin antigen (HA) of avian influenza virus (AIV) is an immunogen abundant on the surfaces of infected cells, and can be used as a target for specific antibodies to clear viral infection. Protamine has been demonstrated to deliver DNA into cells effectively. Accordingly, a fusion protein of anti-HA single-chain fragment variable (scFv) and truncated protamine (tP) may be used as a v...

2015
Sebastian Miethe Christine Rasetti-Escargueil Arnaud Avril Yvonne Liu Siham Chahboun Hannu Korkeala Christelle Mazuet Michel-Robert Popoff Thibaut Pelat Philippe Thullier Dorothea Sesardic Michael Hust Ellen R Goldman

BACKGROUND Botulinum neurotoxins (BoNTs) are considered to be the most toxic substances known on earth and are responsible for human botulism, a life-threatening disease characterized by flaccid muscle paralysis that occurs naturally by food-poisoning or colonization of the gastrointestinal tract by BoNT-producing clostridia. BoNTs have been classified as category A agent by the Centers of Dise...

Journal: :Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2000
A Q Maranhão M M Brígido

We describe the expression of an anti-Z-DNA single chain variable region antibody fragment (scFv) on a filamentous phage surface. Four vectors for phage display were constructed. Two of them are able to display multiple copies of the antibody fragment, and the others can be used to make monovalent libraries. The vectors use different promoter/leader sequences to direct the expression of the fus...

2002
R. Burioni

Single-chain variable fragments (scFvs) are small tumor-recognition units that hold enormous potential in antibody-based therapeutics. Advantages of scfv over whole antibodies are now explored for radioimmunodetection and for insitu radiotherapy of cancer due to potentially better tumor penetration and blood clearance and reduced immunogenicity. Their clinical applications, however, require the...

2016
Nina Persson Bo Jansson Nicolai Stuhr-Hansen András Kovács Charlotte Welinder Lena Danielsson Ola Blixt

We have developed a combinatory antibody-antigen microarray for direct screening of multiple single-chain fragment variable (scFv) clones with no need for pre-purification or enrichment before screening. The straightforward workflow allows for early selection of binders to predefined peptide and glycopeptide targets. A capture antibody is contact printed on microarray slides, side by side with ...

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