Three major and two minor species of ovomucoid were separated by chromatography on sulphoethyl-Sephadex. The predominant sialic acid-free species was further resolved into three fractions by DEAE-cellulose chromatography. Although all species of ovomucoid had closely similar trypsin-inhibiting activity, immunochemical properties and amino acid composition, they differ in carbohydrate compositio...

In our previous investigations, two sialic acids were isolated from the jelly coat of the eggs of the sea urchin Pseudocentrotus depepr~ssl~s; one was N-glycolylneuraminic arid, the other was W-acctoglycolyl-4-methyl-4,9-dideosyncuraminic acid (2). The presence of additional sialic acid compounds was detected in the mixt’urc resulting from mild acid hydrolysis of the starting material (1). Isol...

A density tunable dendrimeric array was designed for assembly of a gold nanocluster probe, which was unloaded by its chemoselective recognition to cell surface sialic acid for in situ tracing of sialic acid density.

1. The total sialic acid content of human gastric aspirates was measured using a colorimetric assay. Care was taken to optimize the assay and to eliminate interference. 2. The sialic acid content of gastric aspirates collected under resting conditions from 77 patients with non-ulcer dyspepsia was found to decrease with age from > 100 micrograms/ml at 25 years and younger to < 20 micrograms/ml a...

Subambient pressure ionization with nanoelectrospray (SPIN) has proven to be effective in producing ions with high efficiency and transmitting them to low pressures for increased sensitivity in mass spectrometry (MS) analysis. Here we present evidence that the SPIN source not only improves MS sensitivity but also facilitates the detection of more labile compounds. The gentleness of conventional...

Reports of influenza A virus infections in dogs has received considerable attention from veterinarians, virologists, and epidemiologists. Interaction between influenza viral hemagglutinin and cell oligosaccharides containing sialic acid residues results in infection. Sialic acids have an α-2,3-linkage to the penultimate galactose in the avian influenza virus receptor and an α-2,6-linkage in the...