Plasmid-mediated carbapenem-hydrolyzing β-lactamases are becoming emerging threats with Enterobacteriaceae. In particular, the carbapenem-hydrolyzing class D β-lactamase OXA-48 and its derivative OXA-181 have been reported increasingly worldwide. Using a PCR-based strategy, environmental samples were screened for bla(OXA-48)-like genes. Shewanella xiamenensis, an environmental species from mari...

DNA microarrays constructed with full length ORFs from Shewanella oneidensis, MR-1, were hybridized with genomic DNA from nine other Shewanella species and Escherichia coli K-12. This approach enabled visualization of relationships between organisms by comparing individual ORF hybridizations to 164 genes and is further amenable to high-density high-throughput analyses of complete microbial geno...

Three different approaches were employed to improve single chambered microbial fuel cell (sMFC) performance using Shewanella putrefaciens as biocatalyst. Taguchi design was used to identify the key process parameter (anolyte concentration, CaCl₂ and initial anolyte pH) for maximization of volumetric power. Supplementation of CaCl₂ was found most significant and maximum power density of 4.92 W/m...

Dissimilatory metal reducing organisms play key roles in the biogeochemical cycle of metals as well as in the durability of submerged and buried metallic structures. The molecular mechanisms that support electron transfer across the microbe-metal interface in these organisms remain poorly explored. It is known that outer membrane proteins, in particular multiheme cytochromes, are essential for ...

Bacterial reduction of arsenic(V) and iron(III) oxides influences the redox cycling and partitioning of arsenic (As) between solid and aqueous phases in sediment-porewater systems. Two types of anaerobic bacterial incubations were designed to probe the relative order of As(V) and Fe(III) oxide reduction and to measure the effect of adsorbed As species on the rate of iron reduction, using hydrou...

Fe(III)-respiring bacteria such as Shewanella species play an important role in the global cycle of iron, manganese, and trace metals and are useful for many biotechnological applications, including microbial fuel cells and the bioremediation of waters and sediments contaminated with organics, metals, and radionuclides. Several alternative electron transfer pathways have been postulated for the...

Project Goals: This project is a component of the Shewanella Federation and as such contributes to the overall goal of applying the tools of genomics, leveraging the availability of genome sequence for 18 additional strains of Shewanella, to better understand the ecophysiology and speciation of respiratory-versatile members of this important genus. To understand these systems the SF is using ge...

A novel halophilic and metal-reducing bacterium, Orenia metallireducens strain Z6, was isolated from briny groundwater extracted from a 2.02 km-deep borehole in the Illinois Basin, IL. This organism shared 96% 16S rRNA gene similarity with Orenia marismortui but demonstrated physiological properties previously unknown for this genus. In addition to exhibiting a fermentative metabolism typical o...