1. Normal serum, used in cultures for preparation of the streptolysin, modifies the properties of the streptolysin, causing delayed hemolysis, increased filterability, resistance to oxidation or reduction, and absence of antigenicity. 1. Streptolysin prepared without serum is an active antigen. 3. Similar temperatures are required to destroy the antigenic activity of serum-free streptolysin and...